pCJH7_pETcc2-EcG3PD-CaNOX vector (V016255)

LOCUS       V016255                 4941 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V016255
VERSION     V016255
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4941)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..4941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1018
                     /gene="gpsA"
                     /label="Glycerol-3-phosphate dehydrogenase [NAD(P)+]"
                     /note="Glycerol-3-phosphate dehydrogenase [NAD(P)+] from
                     Escherichia coli (strain SMS-3-5 / SECEC). Accession#:
                     B1LK47"
     terminator      2446..2493
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(2859..2887)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        3000..3104
                     /label="AmpR promoter"
     CDS             3105..3962
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4136..4724
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4804..4822
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4854..4876
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4895..4912
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4922..4939
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"