LOCUS       V016255                 4941 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V016255
VERSION     V016255
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4941)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..4941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1018
                     /gene="gpsA"
                     /label="Glycerol-3-phosphate dehydrogenase [NAD(P)+]"
                     /note="Glycerol-3-phosphate dehydrogenase [NAD(P)+] from
                     Escherichia coli (strain SMS-3-5 / SECEC). Accession#:
                     B1LK47"
     terminator      2446..2493
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(2859..2887)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        3000..3104
                     /label="AmpR promoter"
     CDS             3105..3962
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4136..4724
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4804..4822
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4854..4876
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4895..4912
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4922..4939
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
ORIGIN
        1 tatgaatcag cgtaatgcaa gcatgaccgt tattggtgca ggtagctatg gcaccgcact
       61 ggcaattacc ctggcacgta atggtcatga agttgttctg tggggtcatg atccggaaca
      121 cattgcaacc ctggaacgtg atcgttgtaa tgcagcattt ctgccggatg ttccgtttcc
      181 ggataccctg catctggaaa gcgatctggc aacagcactg gcagcaagcc gtaatattct
      241 ggttgttgtt ccgagccatg tttttggtga agttctgcgt cagattaaac cgctgatgcg
      301 tccggatgca cgtctggttt gggcaaccaa aggtctggaa gcagaaaccg gtcgtctgct
      361 gcaggatgtt gcacgtgaag cactgggtga tcagattccg ctggcagtta ttagcggtcc
      421 gacctttgca aaagaactgg cagcaggtct gccgaccgca attagcctgg caagcaccga
      481 tcagaccttt gcagatgatc tgcagcaact gctgcattgt ggtaaaagct ttcgtgttta
      541 tagcaacccg gattttattg gtgttcagct gggtggtgca gtgaaaaatg ttattgcaat
      601 tggtgccggt atgagtgatg gtattggttt tggtgcaaat gcacgtaccg cactgattac
      661 ccgtggtctg gcagaaatga gccgtctggg tgcagcactg ggtgcagatc cggcaacctt
      721 tatgggtatg gcaggtctgg gtgatctggt tctgacctgt accgataatc agagccgtaa
      781 tcgtcgtttt ggtatgatgc tgggtcaggg tatggatgtt cagagcgcac aagaaaaaat
      841 tggtcaggtt gttgaaggtt accgcaatac caaagaagtg cgcgaactgg cacatcgttt
      901 tggtgttgaa atgccgatta ccgaagaaat ctatcaggtt ctgtattgcg gcaaaaatgc
      961 ccgtgaagca gcactgaccc tgctgggtcg tgcacgtaaa gatgaacgta gcagccatgg
     1021 tagcagcggt agcagtggta gctcaggtag tagcggttca agcggtagca gcatgaaaat
     1081 tgttgttatt ggttgtaccc atgcaggcac cgcagcagtt aaaaccattc tgaaagaaaa
     1141 tccggaagcc gaaatcacca tttttgaacg caatgacaac atcagctttc tgagctgtgg
     1201 tattgcactg tatgttggtg gtgttgttaa agatccggca ggtctgtttt atagtaatcc
     1261 ggaagaactg agcaaaatgg gtgccaatgt taaaatcaaa cataatgtga aaagcattga
     1321 taccaaaagc aaaaaagtga tcgccgagga tatgaatacc ggtgaagaaa ttgaagtgag
     1381 ctatgacaaa ctggttaata ccaccggtag ctggccgatt attccgccta ttccgggtat
     1441 tgaaagcaaa aacattctgc tgtgcaaaaa ctatgatcag gccaatgtta ttatccgcca
     1501 gaccaaagat gccaaaaaaa tcgttattgt gggtggtggc tatattggta ttgaactggt
     1561 tgaagcattt cagaaaagcg gtaaacaggt taccctgatt gatggtctgg atcgtattct
     1621 gaacaaatac ctggataaag agttcaccga tatcctggaa gatgatctga aaaaaaacgg
     1681 cattaatctg gcactggatc agtgcgttaa aagctttaaa gccaatgaaa atggtgaagt
     1741 gaccagcgtt gaaaccacca aaggtgaata tgaagccgat atggttattc tgtgtgttgg
     1801 ttttcgtccg aataacgaac tgctgaaagg taaagttgat atgctgccga atgatgccat
     1861 tatcgtggat gaatatatgc gtaccagcga tccggatatt tttgcagccg gtgatagctg
     1921 tgcagttcat tataatccga atggcaacta tgcatatatt ccgctggcca ccaatgcagt
     1981 tcgtatgggt atgctgattg gtaaaaacat tagcaccccg aaagttaaat atcgtggcac
     2041 ccagagcacc agcggtctga acctgtttgg ttataacatt ggtagcaccg gtgttaccgt
     2101 tagtggtgca ccgcagattg gtctgaatgt tcgtagcgtt attgtgaaag ataactaccg
     2161 tccggaattt atgccgacca atgaagaaat tatcatgcag ctggtttatg aagttggcac
     2221 caatcgtatt gttggtggtc aggttatgag caaatatgat attacccaga gcgcaaatac
     2281 cctgagcctg gcaattcaga ataaaatgac cattgaagat ctggcctatg tggatttttt
     2341 ctttcagccg cattttgatc gtccgtggaa ttatctgaat attctgggtc tggcagcgtt
     2401 ggaacaagaa ggtctgtaat tgggatccga tccgctgagc aataactagc ataacccctt
     2461 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
     2521 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
     2581 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     2641 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     2701 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     2761 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc
     2821 ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag
     2881 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     2941 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3001 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3061 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3121 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3181 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3241 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3301 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     3361 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3421 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3481 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3541 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3601 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     3661 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4261 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4741 cctttttacg gttcctggcc ttttgctggc cttttgctca catggatctc gatcccgcga
     4801 aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
     4861 aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc acagcagcgg
     4921 cctggtgccg cgcggcagcc a
//
