Basic Vector Information
- Vector Name:
- pcW619
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8456 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Zhou Y.
pcW619 vector Map
pcW619 vector Sequence
LOCUS V016243 8456 bp DNA circular SYN 15-JUN-2020
DEFINITION Exported.
ACCESSION V016243
VERSION V016243
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8456)
AUTHORS Zhou Y.
TITLE Direct Submission
JOURNAL Submitted (24-SEP-2019) Department of Public Health Laboratory
Sciences, West China School of Public Health, Sichuan University,
No. 17, Section 3, Renmin South Road, Wuhou District, Chengdu,
Sichuan 610041, China
REFERENCE 2 (bases 1 to 8456)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(24-SEP-2019) Department of Public Health Laboratory Sciences, West
China School of Public Health, Sichuan University, No. 17, Section
3, Renmin South Road, Wuhou District, Chengdu, Sichuan 610041,
China"
FEATURES Location/Qualifiers
source 1..8456
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 515..703
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 808..948
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1134..1722)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1896..2753)
/label="AmpR"
/note="beta-lactamase"
promoter complement(2754..2858)
/label="AmpR promoter"
CDS 6136..6867
/gene="ermC"
/label="rRNA adenine N-6-methyltransferase"
/note="rRNA adenine N-6-methyltransferase from
Staphylococcus aureus. Accession#: P02979"
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