LOCUS       V016243                 8456 bp    DNA     circular SYN 15-JUN-2020
DEFINITION  Exported.
ACCESSION   V016243
VERSION     V016243
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Zhou Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2019) Department of Public Health Laboratory
            Sciences, West China School of Public Health, Sichuan University,
            No. 17, Section 3, Renmin South Road, Wuhou District, Chengdu,
            Sichuan 610041, China
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-SEP-2019) Department of Public Health Laboratory Sciences, West
            China School of Public Health, Sichuan University, No. 17, Section
            3, Renmin South Road, Wuhou District, Chengdu, Sichuan 610041,
            China"
FEATURES             Location/Qualifiers
     source          1..8456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             6136..6867
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga
       61 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      121 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      181 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      241 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
      301 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
      361 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
      421 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
      481 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct
      541 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
      601 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
      661 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
      901 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
      961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     2221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     2521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca
     3001 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt
     3061 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca
     3121 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct
     3181 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt
     3241 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa
     3301 atgaaaaact ccgcagatac acttaaaaaa gttctagagc aagttaagga agtagcgctg
     3361 acgtgattaa ggtagagttt attttcccct gaagattgtt tgaaagcttg atgagcagtt
     3421 tggacaattt cgttgtattt agttttaatg gtaggtccat taaagttatc ttgaataact
     3481 tgattttctg cagcgaactt cattccgaat ttgctatagt taatagttaa tggtttatta
     3541 gtgccgttct ctaaaagtag caaaatttta ccgcggagct cttttagtgt agggaattta
     3601 tcgctagaat ctgattttgg agttgtgtag aaaaatgctt tgtactgatt aattaaaggt
     3661 tggattcgat aatcaaatct atcgttttta tgattatttt catcttttaa tcgcataata
     3721 atggtttctt tagggtgttc ttttaaaaag ttaaccgttg tttctaatac tcctttaagt
     3781 gaagcgtcta aatagatagg cccatgataa atttggaggt cctctttcgc tctaatatca
     3841 atgtatcgta tcccggcctc tagttgttga aatagcgaca tcttttgggt ttgggcaagg
     3901 gcttttgtga gcgtccagct gatattccct ttataactca tggtgtcatg tgttccaggg
     3961 atagtaagtg aagttaattt cgtattgtca ggcagggctg acatccattg cttagtagtt
     4021 atagaacttt gtgcagttct ataactgttt ttaattgagt gcgcttttcc ggctgatgga
     4081 aaagtaataa tgtaacaaac taaacataat aatactacta aaacttggcg tgaacgaagc
     4141 ttatacatta ttatttcctc ctttgatttg tataagccta tcttataaat aatttcatat
     4201 cgaatcttta acatttgtta aagacgttaa agtttactag atttacaata aaaccataaa
     4261 caaaggatat aatattgcgt ttctaaatct tggaagcgaa tctcgtcaat attatattgg
     4321 aaggagaggg gtggaacata ctacttggca ttattaggtt aaaaaatgta gaaggagagt
     4381 gaaaaccgcg gccgctaaaa atatggaaaa ccccccgctt atcacacaca cataagcttg
     4441 tgagacattc gtcgcgaatc atttggggcg aatgtcgaaa caagcttatt attttttgag
     4501 ttttaacaaa tgtcaaagaa tgggatgctt cttattcata taatataagc atattaaaag
     4561 tttgagtcac ttatagtttc ctactgcgaa aacatataat ttagttccca cccatactaa
     4621 aattgtgtcc ttaactctct attaatttgg ctatacgtgg cttaatcttt taattacata
     4681 atttttgtag cattaaaatt gcgaaaggag agactgtatg aaaagaaaaa caatttttct
     4741 aattttttta ttagtctttg tgggttattt taatgtaaag gtaaaagcgg actctgttga
     4801 aaaagaaaag cttcaaagtg atatacaatc cgaaatgaaa gcaatagatt tacaagcttt
     4861 accaaattgc tacaaggcaa aaagcgatta caagaatttg aaaattacta atagtgaaaa
     4921 agatagcatg ggaatcacac atattacact tgcgatgaat tccgatggtt attttacaga
     4981 tcacgatgaa ataaaactcc atattagccc agaaaataaa attctcttta taaatggcga
     5041 tctaaaacaa acgcggccta ctattacaaa taaaatgaaa cttacagaac aagatgcgat
     5101 agaaaaggca tttgaagcaa tcggacgaaa tgaagcaagt gtcaatagct acataggaag
     5161 cccgattaaa gaaaaaagag taatcgtaaa ttcaaggaca aagcgtttag tatatagtat
     5221 aaggttgatt ttttctgaac cagtagtagc tagctggatt attcagattg acgctgagac
     5281 gggtgcagtt ttacgaaaac agaatatgct atcggaagct aactctcaag ggacgaaaaa
     5341 aaatattata gctccaggaa aaggatacga tccatcatta caaagaccat tgaatgtttt
     5401 gaagataaag aactttttct gcctagttga cagaacccgc aaaggactaa taagaacgtt
     5461 tgatttgaac cataaactag tcccggggcg aattgcatct gaccgaattt tacgtttccc
     5521 tgaataattc tcatcaatcg tttcatcaat tttatcttta tactttatat tttgtgcgtt
     5581 aatcaaatca taatttttat atgtttcctc atgatttatg tctttattat tatagttttt
     5641 attctctctt tgattatgtc tttgtatccc gtttgtatta cttgatcctt taactctggc
     5701 aaccctcaaa attgaatgag acatgctaca cctccggata ataaatatat ataaacgtat
     5761 aaagatttca taaagtctaa cacactagac ttatttactt cgtaattaag tcgttaaacc
     5821 gtgtgctcta cgaccaaaac tataaaacct ttaagaactt tcttttttta caagaaaaaa
     5881 gaaattagat aaatctctca tatcttttat tcaataatcg catccgattg cagtataaat
     5941 ttaacgatca ctcatcatgt tcatatttat cagagctcgt gctataatta tactaatttt
     6001 ataaggagga aaaaatatgg gcatttttag tatttttgta atcagcacag ttcattatca
     6061 accaaacaaa aaataagtgg ttataatgaa tcgttaataa gcaaaattca tataaccaaa
     6121 ttaaagaggg ttataatgaa cgagaaaaat ataaaacaca gtcaaaactt tattacttca
     6181 aaacataata tagataaaat aatgacaaat ataagattaa atgaacatga taatatcttt
     6241 gaaatcggct caggaaaagg ccattttacc cttgaattag taaagaggtg taatttcgta
     6301 actgccattg aaatagacca taaattatgc aaaactacag aaaataaact tgttgatcac
     6361 gataatttcc aagttttaaa caaggatata ttgcagttta aatttcctaa aaaccaatcc
     6421 tataaaatat atggtaatat accttataac ataagtacgg atataatacg caaaattgtt
     6481 cttgatagta tagctaatga gatttattta atcgtggaat acgggtttgc taaaagatta
     6541 ttaaatacaa aacgctcatt ggcattactt ttaatggcag aagttgatat ttctatatta
     6601 agtatggttc caagagaata ttttcatcct aaacctaaag tgaatagctc acttatcaga
     6661 ttaagtagaa aaaaatcaag aatatcacac aaagataaac aaaagtataa ttatttcgtt
     6721 ctgaaatggg ttaacaaaga atacaagaaa atatttacaa aaaatcaatt taacaattcc
     6781 ttaaaacatg caggaattga cgatttaaac aatattagct ttgaacaatt cttatctctt
     6841 gtcaatagct ataaattatt taataagtaa gttaagggat gcataaactg catcccttaa
     6901 cttgtttttc gtgtgcctat tttttgtgaa tcgtcgacaa gctttaatgc ggtagtttat
     6961 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg
     7021 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
     7081 cgggcctctt gcgggatcga tagaaagcgt gagaaacagc gtacagacga tttagagatg
     7141 tagaggtact tttatgccga gaaaactttt tgcgtgtgac agtccttaaa atatacttag
     7201 agcgtaagcg aaagtagtag cgacagctat taactttcgg ttgcaaagct ctaggatttt
     7261 taatggacgc agcgcatcac acgcaaaaag gaaattggaa taaatgcgaa atttgagatg
     7321 ttaattaaag acctttttga ggtctttttt tcttagattt ttggggttat ttaggggaga
     7381 aaacataggg gggtactacg acctcccccc taggtgtcca ttgtccattg tccaaacaaa
     7441 taaataaata ttgggttttt aatgttaaaa ggttgttttt tatgttaaag tgaaaaaaac
     7501 agatgttggg aggtacagtg atagttgtag atagaaaaga agagaaaaaa gttgctgtta
     7561 ctttaagact tacaacagaa gaaaatgaga tattaaatag aatcaaagaa aaatataata
     7621 ttagcaaatc agatgcaacc ggtattctaa taaaaaaata tgcaaaggag gaatacggtg
     7681 cattttaaac aaaaaaagat agacagcact ggcatgctgc ctatctatga ctaaattttg
     7741 ttaagtgtat tagcaccgtt attatatcat gagcgaaaat gtaataaaag aaactgaaaa
     7801 caagaaaaat tcaagaggac gtaattggac atttgtttta tatccagaat cagcaaagcc
     7861 gagtggttag agtatttaaa agagttacac attcaatttg tagtgctcca ttacatgata
     7921 gggatactga tacagaaggt aggatgaaaa aagagcatta tcatattcta gtgatgtatg
     7981 agggtaataa atcttatgaa cagataaaaa taataacaga agaattgaat gcgactattc
     8041 cgcagattgc aggaagtgtg aaaggtcttg tgagatatat gcttcacatg gacgatccta
     8101 ataaatttaa atatcaaaaa gaagatatga tagtttatgg cggtgtagat gttgatgaat
     8161 tattaaagaa aacaacaaca gatagatata aattaattaa agaaatgatt gagtttattg
     8221 atgaacaagg aatcgtagaa tttaagagtt taatggatta tgcaatgaag tttaaatttg
     8281 atgattggtt cccgctttta tgtgataact cggcgtatgt tattcaagaa tatataaaat
     8341 caaatcggta taaatctgac cgatagattt tgaatttagg tgtcacaaga cactcttttt
     8401 tcgcaccagc gaaaactggt ttaagccgag tgcgcaaaag acataatcgg gaattc
//
