pG20_LUC2_Hyg vector (V016232)

LOCUS       62056_11155        7171 bp DNA     circular SYN 03-NOV-2020
DEFINITION  Cloning vector pG20_LUC2_Hyg, complete sequence.
ACCESSION   MT896408
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Pratt AI, Knoblauch J, Kunz H-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in 
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Pratt AI, Kunz H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington 
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
REFERENCE   3  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2020) School of Biological Sciences, Washington State 
            University, 1150 NE College Ave #385, Pullman, WA 99163, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(655..831)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1066..2088)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(2108..2453)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      2463..3096
                     /label=pUBQ10
                     /note="pUBQ10"
                     /regulatory_class="promoter"
     misc_feature    3103..3180
                     /label=MCS
                     /note="MCS"
     CDS             3181..4830
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4841..5089
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    5254..5278
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5369..5957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6131..6943)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"