LOCUS       62056_11155        7171 bp DNA     circular SYN 03-NOV-2020
DEFINITION  Cloning vector pG20_LUC2_Hyg, complete sequence.
ACCESSION   MT896408
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Pratt AI, Knoblauch J, Kunz H-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in 
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Pratt AI, Kunz H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington 
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
REFERENCE   3  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2020) School of Biological Sciences, Washington State 
            University, 1150 NE College Ave #385, Pullman, WA 99163, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(655..831)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1066..2088)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(2108..2453)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      2463..3096
                     /label=pUBQ10
                     /note="pUBQ10"
                     /regulatory_class="promoter"
     misc_feature    3103..3180
                     /label=MCS
                     /note="MCS"
     CDS             3181..4830
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4841..5089
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    5254..5278
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5369..5957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6131..6943)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
      541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
      601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg
      661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
      721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag
      781 aaccctaatt tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga
      841 gatagatttg tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg
      901 cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc
      961 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
     1021 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta ctctattcct ttgccctcgg
     1081 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     1141 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     1201 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     1261 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccttg gcgatcctgc
     1321 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     1381 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     1441 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     1501 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     1561 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     1621 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     1681 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatagcat ccatagcctc
     1741 cgcgaccggc tgaagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     1801 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac
     1861 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     1921 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     1981 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     2041 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatag gggggatcag
     2101 cttgggctgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     2161 gatagtggga ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa tccacttgct
     2221 ttgaagtcgt ggttggaacg acttcttttt ccacgatgct cctcgtgggt gggggtccat
     2281 ctttgggacc actgtcggca gaggcatctt gaatgatagc ctttccttta tcgcaatgat
     2341 ggcatttgta ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg
     2401 ggcaatggaa tccgaggagg tttcccgaaa ttaccctttg ttgaaaagtc tcaataaagc
     2461 ttcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt
     2521 ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa
     2581 caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca
     2641 ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa
     2701 caatacccaa agacctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa
     2761 actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca
     2821 aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc
     2881 tgttaatctt agatcgaagt cgattttctg ggtttgatcg ttagatatca tcttaattct
     2941 cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa
     3001 ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg
     3061 aatttgtcga ttaatctgag tttttctgat taacagacta gagttaaccg ggctcaggcc
     3121 tggcgcgcca ctagtggatc catcgatagt actgtcgacc tcgagggtac cgctcccggg
     3181 atggaagatg ctaagaatat taagaaggga ccagctcctt tctacccact cgaagatgga
     3241 acagcaggtg aacagttaca caaggctatg aaaagatatg ctcttgttcc tggtactatc
     3301 gcattcacag atgctcatat cgaggtggat ataacttatg ctgagtactt cgaaatgtct
     3361 gttaggctcg ctgaagctat gaagagatac ggattaaata caaaccacag gattgttgtg
     3421 tgtagtgaaa attctttgca atttttcatg cctgttcttg gagctttgtt catcggtgtg
     3481 gctgttgcac cagctaatga tatatataac gagagagaac ttcttaattc tatgggaatt
     3541 tctcaaccta ctgttgtgtt cgtgtctaag aaaggacttc aaaagatttt gaacgttcag
     3601 aagaaacttc caattatcca gaagattatt attatggatt caaagacaga ttaccaaggt
     3661 ttccagtcaa tgtacacctt tgtgactagt catttgcctc ctggattcaa tgagtacgat
     3721 ttcgttccag aaagttttga tagagataag actattgctc tcattatgaa ctcttcagga
     3781 tctacaggtc ttcctaaggg tgtggcattg ccacatagaa ccgcttgtgt taggttctct
     3841 cacgcaagag atcctatttt cggaaaccag ataattccag atacagctat cttgtcagtt
     3901 gtgcctttcc atcacggatt tggaatgttc actacactcg gataccttat ttgtggtttt
     3961 agggttgtgc tcatgtacag attcgaagag gaactctttt taagatcttt acaagattat
     4021 aagatacagt cagcactctt agttccaacc cttttctctt tctttgctaa gtcaactttg
     4081 atcgataagt acgatctttc taacttgcat gagattgcaa gtggaggtgc tcctctttct
     4141 aaagaagtgg gtgaagcagt tgctaagagg tttcacttgc caggaatcag acaaggatat
     4201 ggtcttacag agaccacttc tgctatcttg ataacccctg aaggagatga taaaccagga
     4261 gcagttggaa aggttgttcc tttctttgaa gctaaagttg tggatctcga tacaggaaag
     4321 accttaggtg tgaatcagag gggagagctt tgcgttagag gtcctatgat aatgagtgga
     4381 tatgttaata acccagaagc aactaacgct cttatcgata aagatggatg gttgcattca
     4441 ggagatattg cttactggga tgaggatgaa cactttttca tagtggatag gctcaagtca
     4501 ttaattaagt ataagggata ccaagttgca cctgctgagc tcgaaagtat tcttttgcag
     4561 catccaaaca tcttcgatgc aggagttgct ggtcttcctg atgatgatgc tggagagctc
     4621 ccagctgcag ttgtggtttt agaacacgga aagactatga cagagaaaga aattgtggat
     4681 tacgttgcat ctcaagttac aaccgctaag aaacttagag gtggtgtggt ttttgtggat
     4741 gaggttccaa agggactcac tggtaaatta gatgctagaa agatcaggga aattttgatc
     4801 aaagcaaaga aaggaggaaa gattgctgtt tgagagctca tatgaagatg aagatgaaat
     4861 atttggtgtg tcaaataaat agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt
     4921 gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatctca ttataatgaa
     4981 taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct gcagcatata
     5041 actactgtat gtgctatggt atggactatg gaatatgatt aaagataagg aattcagatc
     5101 ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc
     5161 cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg atcagattgt
     5221 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacta
     5281 agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa aaggccagga
     5341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5821 gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc tcttgatccg
     5881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgtg taacattggt
     6121 ctagtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     6181 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     6241 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     6301 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     6361 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     6421 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     6481 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     6541 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     6601 caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc
     6661 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     6721 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     6781 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt
     6841 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     6901 atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     6961 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     7021 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     7081 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     7141 tcaaaatcac caactggtcc acctacaaca a
//
