Basic Vector Information
- Vector Name:
- pMEK19
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4551 bp
- Type:
- Transformation vector
- Replication origin:
- ori
- Source/Author:
- Yu Q, LaManna L, Kelly ME, Lutz KA
pMEK19 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMEK19 vector Sequence
LOCUS 62056_16735 4551 bp DNA circular SYN 08-SEP-2019 DEFINITION Transformation vector pMEK19, complete sequence. ACCESSION MN326018 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4551) AUTHORS Yu Q, LaManna L, Kelly ME, Lutz KA, Maliga P. TITLE New Tools for Engineering the Arabidopsis Plastid Genome JOURNAL Plant Physiol. (2019) In press PUBMED 31427463 REFERENCE 2 (bases 1 to 4551) AUTHORS Maliga P, Kelly ME. TITLE Direct Submission JOURNAL Submitted (14-AUG-2019) Waksman Institute of Microbiology, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA REFERENCE 3 (bases 1 to 4551) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Physiol. (2019) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (14-AUG-2019) Waksman Institute of Microbiology, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4551 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..1055 /note="left plastid targeting region of vector, A. thaliana RLD plastid DNA" misc_feature complement(1..596) /label=16S ribosomal RNA fragment /note="16S ribosomal RNA fragment" tRNA complement(828..899) /product="tRNA-Val" misc_feature complement(1063..1119) /label=MCS /note="pUC18/19 multiple cloning site" misc_feature 1131..1980 /note="right plastid targeting region of vector, A. thaliana RLD intergenic plastid DNA" promoter complement(1990..2008) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2015..2031) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 2173..2628 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2654..2758 /label=AmpR promoter CDS 2759..3616 /label=AmpR /note="beta-lactamase" rep_origin 3790..4378 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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