LOCUS       62056_16735        4551 bp DNA     circular SYN 08-SEP-2019
DEFINITION  Transformation vector pMEK19, complete sequence.
ACCESSION   MN326018
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Yu Q, LaManna L, Kelly ME, Lutz KA, Maliga P.
  TITLE     New Tools for Engineering the Arabidopsis Plastid Genome
  JOURNAL   Plant Physiol. (2019) In press
  PUBMED    31427463
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Maliga P, Kelly ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2019) Waksman Institute of Microbiology, Rutgers 
            University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
REFERENCE   3  (bases 1 to 4551)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-AUG-2019) Waksman Institute of Microbiology, Rutgers University,
            190 Frelinghuysen Road, Piscataway, NJ 08854, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1055
                     /note="left plastid targeting region of vector, A. thaliana
                     RLD plastid DNA"
     misc_feature    complement(1..596)
                     /label=16S ribosomal RNA fragment
                     /note="16S ribosomal RNA fragment"
     tRNA            complement(828..899)
                     /product="tRNA-Val"
     misc_feature    complement(1063..1119)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1131..1980
                     /note="right plastid targeting region of vector, A.
                     thaliana RLD intergenic plastid DNA"
     promoter        complement(1990..2008)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2015..2031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2173..2628
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2654..2758
                     /label=AmpR promoter
     CDS             2759..3616
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3790..4378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggtagtttc caccgcctgt ccagggttga gccctgggat ttgacggcgg acttaaaaag
       61 ccacctacag acgctttacg cccaatcatt ccggataacg cttgcatcct ctgtattacc
      121 gcggctgctg gcacagagtt agccgatgct tattccccag ataccgtcat tgcttcttct
      181 ctgggaaaag aagttcagga cccgtaggcc ttctacctcc acgcggcatt gctccgtcag
      241 gctttcgccc attgcggaaa attccccact gctgcctccc gtaggagtct gggccgtgtc
      301 tcagtcccag tgtggctgat catcctctcg gaccagctac tgatcatcgc cttggtaagc
      361 tattgcctca ccaactagct aatcagacgc gagcccctcc tcgggcggat tcctcctttt
      421 gctcctcagc ctacggggta ttagcagccg tttccagctg ttgttcccct cccaagggca
      481 ggttcttacg cgttactcac ccgtccgcca ctggaaacac cacttcccgt ccgacttgca
      541 tgtgttaagc atgccgccag cgttcatcct gagccaggat cgaactctcc atgagattca
      601 tagttgcatt acttatagct tccttcttcg tagacaaagc tgattcggaa ttgtctttca
      661 ttccaagtca taacttgtat ccatgcgctt catattcgca tggagttcgc tcccagaaat
      721 atagctaccc ctaccccctc acgtcaatcc cacgagcctc ttatccattc ttattcgatc
      781 acagcgaggg agcaagtcaa aatagaaaaa ctcacattca ttgggtttag ggataatcag
      841 gctcgaactg atgacttcca ccacgtcaag gtgacactct accgctgagt tatatccctt
      901 cccccatcaa gaaatagaac tgactaatcc taagtcaaag ggtcgagaaa ctcaaggcca
      961 ctattcttga acaacttgga ttggagccgg gctttccttt cgcactttat acgggtatga
     1021 aatgaaaata atggaaaaag ttggattcaa ttgtccgata tcaagcttgc atgcctgcag
     1081 gtcgactcta gaggatcccc gggtaccgag ctcgaattca tttaaatagg actactccta
     1141 tcggaaatag gattgactac ggattcgagc catagcacat ggtttcataa aaccgtacga
     1201 ttctcccgat ctaaatcaag ccggttttac atgaagaaga tttgactcgg catgttctat
     1261 tcgatacggg taggagaaac ggtattcttt tcttaaactt caaaaaatag agaaataaga
     1321 accaagtcaa gatgatacgg attaatcctt tattcttgcg ccaaagatct tcctatttcc
     1381 aaaggaactg gagttacatc tcttttccat ttccattcaa gagttcttat gtgtttccac
     1441 gcccctttaa gaccccgaaa aatcgacaaa ttcccttttc ttaggaccac atgcgagata
     1501 acgaaaaaaa aaaagagaga atggtaaccc cacgattaac tattttattt atgaatttca
     1561 tagtaataga aatacatgtc ctaccgaaac agaatttgta acttgctatc ctatcatctt
     1621 gcctagcagg caaagatttc actccgcgaa aaagatgatt cattcggatc aacatgaaag
     1681 cccaactaca ttgccagaat ttatatattg gaaagaggtt tacctccttg cttctatggt
     1741 acaatcctct tcccgcggag cctcctttct tctcggtccg cagagacaaa atgtaggact
     1801 ggtgccaaca gttaatcacg gaagaaagga ctcactgcgc caagatcact aactaatcta
     1861 atagaataga aaatcctaat ataatagaaa agaaaagaac tgtcttttct gtatacttat
     1921 gtatactttc cccggttccg ttgctactgc gggctttacg caattgatcg gatcatctag
     1981 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     2041 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2101 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2161 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     2221 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     2281 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     2341 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     2401 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2461 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     2521 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2581 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     2641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3001 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3301 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3901 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4441 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4501 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg a
//
