Basic Vector Information
- Vector Name:
- pMK201.2
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 3848 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Hartsough LA, Kotlajich MV, Han B, Lin C-C.J.
pMK201.2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMK201.2 vector Sequence
LOCUS 62056_17015 3848 bp DNA circular SYN 27-JUL-2020 DEFINITION Cloning vector pMK201.2, complete sequence. ACCESSION MN617161 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3848) AUTHORS Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC, Tabor JJ. TITLE Optogenetic control of gut bacterial metabolism JOURNAL Unpublished REFERENCE 2 (bases 1 to 3848) AUTHORS Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC, Tabor JJ. TITLE Direct Submission JOURNAL Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main St., Houston, TX 77030, USA REFERENCE 3 (bases 1 to 3848) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main St., Houston, TX 77030, USA" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3848 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..35 /label=constitutive promoter J23100 /note="constitutive promoter J23100" misc_feature 36..69 /label=B0034 /note="B0034" misc_feature 70..774 /label=ccaR /note="ccaR" terminator 795..866 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 882..909 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 916..1020 /label=pEXT20 expression vector /note="pEXT20 expression vector" misc_feature 1033..1204 /label=PcpcG2-172 /note="PcpcG2-172" misc_feature 1137..1158 /label=G box /note="G box" misc_feature 1205..1224 /label=B0034 /note="B0034" misc_feature 1225..1230 /label=biobricks suffix /note="biobricks suffix" CDS 1231..1851 /gene="rcsA" /label=rcsA /note="Transcriptional regulatory protein RcsA from Escherichia coli (strain K12). Accession#: P0DMC9" terminator complement(1878..1921) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin complement(2191..2779) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(2867..2961) /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2985..3641) /label=CmR /note="chloramphenicol acetyltransferase"
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