LOCUS       V016173                 3848 bp    DNA     circular SYN 27-JUL-2020
DEFINITION  Exported.
ACCESSION   V016173
VERSION     V016173
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Optogenetic control of gut bacterial metabolism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main
            St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3848)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2019) Bioengineering, Rice University, 6500 Main St.,
            Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..3848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..35
                     /label="constitutive promoter J23100"
                     /note="constitutive promoter J23100"
     misc_feature    36..69
                     /label="B0034"
                     /note="B0034"
     misc_feature    70..774
                     /label="ccaR"
                     /note="ccaR"
     terminator      795..866
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      882..909
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    916..1020
                     /label="pEXT20 expression vector"
                     /note="pEXT20 expression vector"
     misc_feature    1033..1204
                     /label="PcpcG2-172"
                     /note="PcpcG2-172"
     misc_feature    1137..1158
                     /label="G box"
                     /note="G box"
     misc_feature    1205..1224
                     /label="B0034"
                     /note="B0034"
     misc_feature    1225..1230
                     /label="biobricks suffix"
                     /note="biobricks suffix"
     CDS             1231..1851
                     /gene="rcsA"
                     /label="Transcriptional regulatory protein RcsA"
                     /note="Transcriptional regulatory protein RcsA from
                     Escherichia coli (strain K12). Accession#: P0DMC9"
     terminator      complement(1878..1921)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2191..2779)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2867..2961)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2985..3641)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctgtta aacgctcctc gtaagaacac
       61 gggaaagcaa tgagaattct tttagtggag gatgatttgc cgctggcgga aacccttgct
      121 gaagcattga gtgaccagct ttacaccgtt gatattgcca ccgacgcttc cctcgcctgg
      181 gactatgcct cccgactgga atatgacctc gttattttgg atgtgatgct gccggagttg
      241 gacgggatta ccctctgtca aaaatggcga tcgcacagtt atttaatgcc aattttgatg
      301 atgacagcca gggatacgat caatgataaa atcacgggct tggatgcggg ggcggatgat
      361 tatgtggtca agccagtgga tttgggggag ttatttgcca gggtgcgagc tttgttgcgt
      421 cggggttgtg caacgtgcca accagtttta gagtgggggc caatcaggtt ggatccaagc
      481 acctatgaag ttagttatga caatgaggtt ttgtctttga cccgcaagga atacagcatt
      541 ctggaattac tactccgcaa tggccgtcgg gtgctaagtc ggagcatgat tatcgatagt
      601 atctggaagt tggagagtcc cccagaggaa gatacggtta aggtgcatgt gcggagtttg
      661 cgacaaaaat taaaaagtgc cggtttatca gcagatgcca ttgaaacggt ccatggcatt
      721 gggtatcgtc tggccaattt aacggaaaaa tctttgtgcc aagggaaaaa ctagtaataa
      781 tctagaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      841 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      901 ctttctgcgt ttatagtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc
      961 tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg
     1021 gttagctagt caagcccatt gtgcttttct ctatcaacct cagcttacct gaaggggtga
     1081 acaggtctgg gttaattcat gttgcgaaat gtaacagttt tagtcgcatc agctaacttt
     1141 ccgatttctt tacgattttc tccccctttt cttcaatttt actttgttag gatcgcattt
     1201 ttaaccttag cctaagaggt atctactagt atgtcaacga ttattatgga tttatgtagt
     1261 tacacccgac taggtttaac cgggtatctg ttgagtagag gggttaaaaa aagagaaatc
     1321 aacgacattg aaaccgttga tgaccttgcc atagcttgtg attcacagcg cccttcagtg
     1381 gtgtttatta atgaggactg tttcatccac gatgcttcta acagtcagcg tatcaagctc
     1441 atcattaatc aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat
     1501 tttgatgaat atctattggt cagaaaaaat ttattgatca gttctaaatc gattaaaccg
     1561 gaatctctcg acgatatcct tggcgatatt ctgaaaaaag agacaacgat aacctcgttt
     1621 ttaaatatgc cgacgttatc attgagccga accgaatcga gtatgttgcg aatgtggatg
     1681 gcaggtcagg gaaccattca aatctctgac caaatgaata tcaaagccaa gaccgtttca
     1741 tcgcataaag gtaatattaa acgtaagatc aaaacgcata ataaacaggt tatctaccat
     1801 gtcgtccgac tgacggataa tgtgactaat ggtatttttg tcaacatgcg ctaataataa
     1861 tctagaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1921 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1981 ctttctgcgt ttataggtac ccgtggatcc tctagagtcg acctgcaggc atgcaagctt
     2041 cctaggcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     2401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     2641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2821 gaacgaaaac tcacgttaag ggattttggt catgactagt gcttggattc tcaccaataa
     2881 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     2941 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     3001 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg
     3061 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     3121 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     3181 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     3241 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     3301 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     3361 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     3421 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     3481 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     3541 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     3601 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     3661 gaaaatctcg ataactcaaa aaatacgacc ggtagtgatc ttatttcatt atggtgaaag
     3721 ttggaacctc ttacgggccg gccaacgtct cattttcgcc agatatcgac gtctaagaaa
     3781 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     3841 acctcgag
//
