7-waxy-guide-CR2 vector (V016171)

Basic Vector Information

Vector Name:
Antibiotic Resistance:
3702 bp
Cloning vector
Replication origin:
Gao H.

7-waxy-guide-CR2 vector Vector Map

7-waxy-guide-CR23702 bp60012001800240030003600attL1gRNA scaffoldattL3T7 promoterM13 revKanRorirrnB T2 terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

7-waxy-guide-CR2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_191        3702 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 7-waxy-guide-CR2, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 3702)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
FEATURES             Location/Qualifiers
     source          1..3702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    21..120
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      204..1203
     misc_RNA        1225..1300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(1372..1467)
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1509..1527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1532..1548)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
     CDS             1661..2467
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2617..3205
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     terminator      complement(3535..3562)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB

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