Basic Vector Information
- Vector Name:
- 7-waxy-guide-CR2
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3702 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Gao H.
7-waxy-guide-CR2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
7-waxy-guide-CR2 vector Sequence
LOCUS 62056_191 3702 bp DNA circular SYN 19-MAR-2020
DEFINITION Cloning vector 7-waxy-guide-CR2, complete sequence.
ACCESSION MN294719
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3702)
AUTHORS Gao H.
TITLE Direct Submission
JOURNAL Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva
Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE 2 (bases 1 to 3702)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience,
8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT ##Assembly-Data-START##
Assembly Method :: Vector NTI v. 10
Sequencing Technology :: Illumina
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3702
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 21..120
/label=attL1
/note="recombination site for the Gateway(R) LR reaction"
regulatory 204..1203
/regulatory_class="promoter"
misc_RNA 1225..1300
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind complement(1372..1467)
/label=attL3
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(1509..1527)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1532..1548)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 1661..2467
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 2617..3205
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(3535..3562)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
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