LOCUS       62056_191        3702 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 7-waxy-guide-CR2, complete sequence.
ACCESSION   MN294719
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    21..120
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      204..1203
                     /regulatory_class="promoter"
     misc_RNA        1225..1300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(1372..1467)
                     /label=attL3
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1509..1527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1532..1548)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1661..2467
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2617..3205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3535..3562)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 aaggccttaa gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt
       61 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct
      121 ccggccagaa tggcccggac cgggttaccg aattcgagct cggtaccctg ggatccgata
      181 tcgatgggcc ctggccgaag ctttgagagt acaatgatga acctagatta atcaatgcca
      241 aagtctgaaa aatgcaccct cagtctatga tccagaaaat caagattgct tgaggccctg
      301 ttcggttgtt ccggattaga gccccggatt aattcctagc cggattactt ctctaattta
      361 tatagatttt gatgagctgg aatgaatcct ggcttattcc ggtacaaccg aacaggccct
      421 gaaggatacc agtaatcgct gagctaaatt ggcatgctgt cagagtgtca gtattgcagc
      481 aaggtagtga gataaccggc atcatggtgc cagtttgatg gcaccattag ggttagagat
      541 ggtggccatg ggcgcatgtc ctggccaact ttgtatgata tatggcaggg tgaataggaa
      601 agtaaaattg tattgtaaaa agggatttct tctgtttgtt agcgcatgta caaggaatgc
      661 aagttttgag cgagggggca tcaaagatct ggctgtgttt ccagctgttt ttgttagccc
      721 catcgaatcc ttgacataat gatcccgctt aaataagcaa cctcgcttgt atagttcctt
      781 gtgctctaac acacgatgat gataagtcgt aaaatagtgg tgtccaaaga atttccaggc
      841 ccagttgtaa aagctaaaat gctattcgaa tttctactag cagtaagtcg tgtttagaaa
      901 ttattttttt atataccttt tttccttcta tgtacagtag gacacagtgt cagcgccgcg
      961 ttgacggaga atatttgcaa aaaagtaaaa gagaaagtca tagcggcgta tgtgccaaaa
     1021 acttcgtcac agagagggcc ataagaaaca tggcccacgg cccaatacga agcaccgcga
     1081 cgaagcccaa acagcagtcc gtaggtggag caaagcgctg ggtaatacgc aaacgttttg
     1141 tcccaccttg actaatcaca agagtggagc gtaccttata aaccgagccg caagcaccga
     1201 attgctggga gagacggtgt agtagtttta gagctagaaa tagcaagtta aaataaggct
     1261 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttttg cggccgcgaa
     1321 ttcctgcagg gccctcttgt cggaccagtt gcccaccacg ttggtgaggg gcaactttat
     1381 tatacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1441 ctatcagtca aaataaaatc attatttgga gctccatgca tggtagcgtt aacgcggccg
     1501 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     1561 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1621 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1681 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1741 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1801 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1861 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1921 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1981 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2041 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2101 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2161 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2221 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2281 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2341 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2401 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2461 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2521 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     2581 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2641 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2701 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2761 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2821 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2881 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2941 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3001 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3061 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3121 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3181 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3241 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3301 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3361 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3421 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3481 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     3541 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     3601 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     3661 ctactcagga gagcgttcac cgacaaacaa cagataaaac ga
//
