pGL4Zc vector (V016165)

LOCUS       62056_11750        4790 bp DNA     circular SYN 31-MAR-2019
DEFINITION  Firefly luciferase reporter vector pGL4Zc, complete sequence.
ACCESSION   MK484108
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Kircher M, Xiong C, Martin B, Schubach M, Inoue F, Bell R, Costello 
            J, Shendure J, Ahituv N.
  TITLE     Saturation mutagenesis of disease-associated regulatory elements
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Kircher M, Xiong C, Martin B, Schubach M, Inoue F, Bell R, Costello 
            J, Shendure J, Ahituv N.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2019) Department of Bioengineering and Therapeutic
            Sciences, CABs and UCSF, 1550 4th street, San Francisco, CA 94158, 
            USA
REFERENCE   3  (bases 1 to 4790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-FEB-2019) Department of Bioengineering and Therapeutic Sciences,
            CABs and UCSF, 1550 4th street, San Francisco, CA 94158, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..504
                     /label=ZRS
                     /note="ZRS"
     CDS             648..2297
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2313..2328
                     /note="sequence was changed from the original sequence;
                     fixed GC stretch"
     polyA_signal    complement(2344..2465)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2884..3472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3675..4532)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4637..4685
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4699..4790
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"