LOCUS       62056_11750        4790 bp DNA     circular SYN 31-MAR-2019
DEFINITION  Firefly luciferase reporter vector pGL4Zc, complete sequence.
ACCESSION   MK484108
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Kircher M, Xiong C, Martin B, Schubach M, Inoue F, Bell R, Costello 
            J, Shendure J, Ahituv N.
  TITLE     Saturation mutagenesis of disease-associated regulatory elements
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Kircher M, Xiong C, Martin B, Schubach M, Inoue F, Bell R, Costello 
            J, Shendure J, Ahituv N.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2019) Department of Bioengineering and Therapeutic
            Sciences, CABs and UCSF, 1550 4th street, San Francisco, CA 94158, 
            USA
REFERENCE   3  (bases 1 to 4790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-FEB-2019) Department of Bioengineering and Therapeutic Sciences,
            CABs and UCSF, 1550 4th street, San Francisco, CA 94158, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..504
                     /label=ZRS
                     /note="ZRS"
     CDS             648..2297
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2313..2328
                     /note="sequence was changed from the original sequence;
                     fixed GC stretch"
     polyA_signal    complement(2344..2465)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2884..3472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3675..4532)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4637..4685
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4699..4790
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagatatggc ttcattttct gtaataaaca ctaagatcaa
       61 aacatgaccc aagttaaatt tccttgcagg gttcccagca ggggcttccc ttttgtctgt
      121 gatttcctct cacccaccag aaccaggcca aatatgcgca tgtgccacta acactaagca
      181 gcacttcctt aatcactcat ttccaacaat ttatggatca tcagtggcaa aaaacgagca
      241 aaaataatga aagaatgcaa tgaaagctcg tggagacaga ggctggactt cctactcact
      301 ctgtgtctct ttaagatgga ggcctgatac aaattagcca ctggggggaa aaagtcatct
      361 ggtcataaaa tacagtacaa ggtcactttt atgtaagttt gccaaaaggg acataaacca
      421 ggacaatttc aaactgtgac acaggataga aacatattaa aaaaatcttt gttcctcctc
      481 tattgtgctg tcatgttgct cagccttaag cttagatcat caagatcgat ctggatccat
      541 gggtgttcct gaaggggggc tataaaaggg ggtgggggcg cgttcgtcct cactctcttc
      601 cgaattcgcg gccagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
      661 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
      721 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
      781 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
      841 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
      901 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
      961 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
     1021 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
     1081 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
     1141 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
     1201 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
     1261 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
     1321 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
     1381 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     1441 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
     1501 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     1561 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     1621 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     1681 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     1741 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     1801 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     1861 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     1921 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     1981 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     2041 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     2101 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     2161 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     2221 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     2281 gcggcaagat cgccgtgtaa taattctaga gtctgagctg acagtcgctt cgagcagaca
     2341 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2401 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2461 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2521 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
     2581 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     2641 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     2701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3301 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
     3661 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
     3721 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
     3781 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
     3841 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
     3901 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
     3961 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
     4021 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
     4081 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
     4141 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
     4201 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
     4261 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
     4321 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
     4381 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
     4441 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
     4501 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
     4561 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
     4621 gtattggaca ggccgcaata aaatatcttt attttcatta cgtccgtgtg ttggtttttt
     4681 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     4741 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
