OX4509 vector (V016161)

LOCUS       62056_1835        9171 bp DNA     circular SYN 10-SEP-2019
DEFINITION  Cloning vector OX4509, complete sequence.
ACCESSION   MK795198
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Haghighat-Khah RE, Harvey-Samuel T, Basu S, StJohn O, Scaife S, 
            Verkuijl S, Lovett E, Alphey L.
  TITLE     Engineered action at a distance: Blood-meal-inducible paralysis in 
            Aedes aegypti
  JOURNAL   PLoS Negl Trop Dis 13 (9), e0007579 (2019)
  PUBMED    31479450
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Haghighat-Khah RE, Scaife S, StJohn O.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2019) Life Sciences, Imperial College London, Sir 
            Alexander Fleming Building, Imperial College Road, London SA7 2AZ, 
            UK
REFERENCE   3  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis"; date: "2019"; volume: "13"; issue: "9"; pages: "e0007579"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2019) Life Sciences, Imperial College London, Sir Alexander 
            Fleming Building, Imperial College Road, London SA7 2AZ, UK"
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(64..185)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1409..1425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1433..1449)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1457..1487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1502..1523)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1811..2399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2573..3430)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3431..3535)
                     /label=AmpR promoter
     primer_bind     4009..4025
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5891..6373
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     CDS             7219..7893
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     CDS             7903..7923
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    8064..8185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8292..8310
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     sig_peptide     8894..8953
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"