LOCUS       62056_1835        9171 bp DNA     circular SYN 10-SEP-2019
DEFINITION  Cloning vector OX4509, complete sequence.
ACCESSION   MK795198
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Haghighat-Khah RE, Harvey-Samuel T, Basu S, StJohn O, Scaife S, 
            Verkuijl S, Lovett E, Alphey L.
  TITLE     Engineered action at a distance: Blood-meal-inducible paralysis in 
            Aedes aegypti
  JOURNAL   PLoS Negl Trop Dis 13 (9), e0007579 (2019)
  PUBMED    31479450
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Haghighat-Khah RE, Scaife S, StJohn O.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2019) Life Sciences, Imperial College London, Sir 
            Alexander Fleming Building, Imperial College Road, London SA7 2AZ, 
            UK
REFERENCE   3  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis"; date: "2019"; volume: "13"; issue: "9"; pages: "e0007579"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2019) Life Sciences, Imperial College London, Sir Alexander 
            Fleming Building, Imperial College Road, London SA7 2AZ, UK"
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(64..185)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1409..1425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1433..1449)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1457..1487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1502..1523)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1811..2399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2573..3430)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3431..3535)
                     /label=AmpR promoter
     primer_bind     4009..4025
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5891..6373
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     CDS             7219..7893
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     CDS             7903..7923
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    8064..8185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8292..8310
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     sig_peptide     8894..8953
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
ORIGIN
        1 ctaggattct agcatgcgca aatttaaagc gctgataaat gagcggcctt aattaactcg
       61 cgttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      181 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      241 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat catcacagga
      301 acaggtggtg gcggccctcg gtgcgctcgt actgctccac gatggtgtag tcctcgttgt
      361 gggaggtgat gtccagcttg gcgtccacgt agtagtagcc gggcagctgc acgggcttct
      421 tggccatgta gatggacttg aactccacca ggtagtggcc gccgtccttc agcttcaggg
      481 ccttgtgggt ctcgcccttc agcacgccgt cgcgggggta caggcgctcg gtggaggggg
      541 ccgatctgac aatgttcagt gcagagactc ggctacgcct cgtggacttt gaagttgacc
      601 aacaatgttt attcttacct ctaatagtcc tctgtggcaa ggtcaagatt ctgttagaag
      661 ccaatgaaga acctggttgt tcaataacat tttgttcgtc taatatttca ctaccgcttg
      721 acgttggctg cacttcatgt acctcatcta taaacgcttc ttctgtatcg ctctggacgt
      781 catcttcact tacgtgatct gatatttcac tgtcagaatc ctcaccaaca agctcgtcat
      841 cgctttgcag aagagcagag aggatatgct catcgtctaa agaactaccc attttattat
      901 atattagtca cgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
      961 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     1021 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     1081 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     1141 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     1201 cgcagactat ctttctaggg ttaaaaaaga tttgcgcttt actcgaccta aactttaaac
     1261 acgtcataga atcttcgttt gacaaaaacc acattgtggc caagctgtgt gacgcgacgc
     1321 gcgctaaaga atggcaaacc aagtcgcgcg agcgtcgacc tgcaggcatg caagcttgca
     1381 tgcctgcagg tcgaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1441 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1501 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1621 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1681 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1741 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1801 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1861 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1921 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1981 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     2041 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2101 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2161 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2221 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2281 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2341 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2401 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2461 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2521 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3241 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3301 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3361 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3421 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3541 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3601 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     3661 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     3721 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     3781 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     3841 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     3901 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3961 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     4021 ccagtgccaa gctttgttta aaatataaca aaattgtgat cccacaaaat gaagtggggc
     4081 aaaatcaaat aattaatagt gtccgtaaac ttgttggtct tcaacttttt gaggaacacg
     4141 ttggacggca aatccgtgac tataacacaa gttgatttaa taattttagc caacacgtcg
     4201 ggctgcgtgt tttttgccga cgcgtctgtg tacacgttga ttaactggtc gattaaactg
     4261 ttgaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt taaaataact
     4321 ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc taatatgagg
     4381 tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt acacgaacgc
     4441 gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg caccatatac
     4501 gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg tgtctctgct
     4561 caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca agtcgatgag
     4621 cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga tatacggtat
     4681 tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc tagaaagata
     4741 atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg tgttttatcg
     4801 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
     4861 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
     4921 aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat tctctctttt
     4981 acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa aataagtaat
     5041 tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag aaacaacttt
     5101 ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg cacgatgcat
     5161 ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt ttcattactg
     5221 gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata ttagagatat
     5281 tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata aacgatgacg
     5341 tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttggaccttt tctcccttgc
     5401 tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt atcattccgt
     5461 acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca gaacacattt
     5521 ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt tgcggtttac
     5581 cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat accatcttag
     5641 ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc
     5701 ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg tttgatcgca
     5761 cggttcccac aatggttgcg gccggccaga tttaaatgag cggccgcatg gtacccattg
     5821 cttgtcattt attaatttgg atgatgtcat ttgtttttaa aattgaactg gctttacgag
     5881 tagaattcta cgcgtaaaac acaatcaagt atgagtcata atctgatgtc atgttttgta
     5941 cacggctcat aaccgaactg gctttacgag tagaattcta cttgtaatgc acgatcagtg
     6001 gatgatgtca tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac
     6061 tcgctttacg agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg
     6121 caatatgata tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt
     6181 agaattctac ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac
     6241 tcgctttacg agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat
     6301 aaaaataaaa gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt
     6361 agaattctac gcgtaaaaca tgattgataa ttaaataatt catttgcaag ctatacgtta
     6421 aatcaaacgg acgctcgagg ttgcacaaca ctattatcga tttgcagttc gggacataaa
     6481 tgtttaaata tatcgatgtc tttgtgatgc gcgcgacatt tttgtaggtt attgataaaa
     6541 tgaacggata cgttgcccga cattatcatt aaatccttgg cgtagaattt gtcgggtcca
     6601 ttgtccgtgt gcgctagcat gcccgtaacg gacctcgtac ttttggcttc aaaggttttg
     6661 cgcacagaca aaatgtgcca cacttgcagc tctgcatgtg tgcgcgttac cacaaatccc
     6721 aacggcgcag tgtacttgtt gtatgcaaat aaatctcgat aaaggcgcgg cgcgcgaatg
     6781 cagctgatca cgtacgctcc tcgtgttccg ttcaaggacg gtgttatcga cctcagatta
     6841 atgtttatcg gccgactgtt ttcgtatccg ctcaccaaac gcgtttttgc attaacattg
     6901 tatgtcggcg gatgttctat atctaatttg aataaataaa cgataaccgc gttggtttta
     6961 gagggcataa taaaagaaat attgttatcg tgttcgccat tagggcagta taaattgacg
     7021 ttcatgttgg atattgtttc agttgcaagt tgacactggc ggcgacaagc aattctaatt
     7081 ggggtaagtt ttcccgttct tttctgggtt cttccctttt gctcatcctt gctgcactac
     7141 cttcaggtgc aagttgagat tcaggccacc atgggagatc ccaccccacc caagaagaag
     7201 cgcaaaccgg tcgccaccat ggcctcctcc gagaacgtca tcaccgagtt catgcgcttc
     7261 aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     7321 ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg cggccccctg
     7381 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
     7441 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
     7501 cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga cccaggactc ctccctgcag
     7561 gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc
     7621 gtgatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     7681 ggcgtgctga agggcgagac ccacaaggcc ctgaagctga aggacggcgg ccactacctg
     7741 gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg ctactactac
     7801 gtggacgcca agctggacat cacctcccac aacgaggact acaccatcgt ggagcagtac
     7861 gagcgcaccg agggccgcca ccacctgttc ctgagatctc gacccaagaa aaagcggaag
     7921 gtggaggacc cgtaagatcc accggatcta gataactgat cataatcagc cataccacat
     7981 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     8041 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     8101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8161 tgtccaaact catcaatgta tcttaacgcg agttaattaa gaggcgcgcc ccggccatcg
     8221 agaaagagag agagaagaga agagagagaa cattcgagaa agagagagag aagagaagag
     8281 agagaacata ctccctatca gtgatagaga agtccctatc agtgatagag atgtccctat
     8341 cagtgataga gagttcccta tcagtgatag agacgtccct atcagtgata gagaagtccc
     8401 tatcagtgat agagagatcc ctatcagtga tagagatttc cctatcagtg atagagaggt
     8461 ccctatcagt gatagagact tccctatcag tgatagagaa atccctatca gtgatagaga
     8521 catccctatc agtgatagag aactccctat cagtgataga gacctcccta tcagtgatag
     8581 agatcgatgc gggcgcatgg tggatgttta aacagcgccg gagtataaat agaggcgctt
     8641 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc
     8701 taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcaggtacc ctggcggtaa
     8761 gttgatcaaa ggaaacgcaa agttttcaag aaaaaacaaa actaatttga tttataacac
     8821 ctttaggcga tcgcccacca tgctgctggt caaccagtcg caccagggat tcaacaaaga
     8881 acacacctcg aagatggtgt cggccatcgt gctgtacgtg ctgctggccg ctgccgccca
     8941 ctcggccttc gccaagaaga acggatacgc cgtcgattcg tcgggaaagg ccccggaatg
     9001 cctgctgtcg aactactgca acaaccagtg taccaaggtg cactacgccg ataagggata
     9061 ctgctgcctg ctgagctgct attgcttcgg actgaacgat gacaagaagg tgctggaaat
     9121 ctcggatacc cgcaagtcgt actgcgatac caccatcatc aactgagcat g
//
