pTH1150.11 vector (V016034)

LOCUS       62056_21185        8108 bp DNA     circular SYN 12-OCT-2020
DEFINITION  Cloning vector pTH1150.11, complete sequence.
ACCESSION   MN872812
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Hammond TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-DEC-2019) School of Biological Sciences, Illinois 
            State University, School of Biological Sciences, Normal, IL 61790, 
            USA
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-DEC-2019) School of Biological Sciences, Illinois State 
            University, School of Biological Sciences, Normal, IL 61790, USA"
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(16..1864,1928..2124)
                     /codon_start=1
                     /transl_table=11
                     /product="HIS-3"
                     /label=HIS-3
                     /note="enzyme in histidine biosynthetic pathway"
                     /protein_id="QOI16730.1"
                     /translation="KLAISTILSSVWKSDRPDGLLPTVVVDEHDTALGLVYSSAESVNE
                     ALRTQTGVYQSRKRGLWYKGATSGDTQELVRISLDCDNDALKFVVKQKGRFCHLDQSGC
                     FGQLKGLPKLEQTLISRKQSAPEGSYTARLFSDEKLVRAKIMEEAEELCTAQTPQEIAF
                     EAADLFYFALTRAVAAGVTLADIERSLDAKSWKVKRRTGDAKGKWAEKEGIKPAASALA
                     ATSAPVTKEAAQETTPEKITMRRFDASKVSTEELDAALKRPAQKSSDAIYKIIVPIIED
                     VRKNGDKAVLSYTHKFEKATSLTSPVLKAPFPKELMQLPEETIAAIDVSFENIRKFHAA
                     QKEEKPLQVETMPGVVCSRFSRPIEAVGCYIPGGTAVLPSTALMLGVPAMVAGCNKIVF
                     ASPPRADGTITPEIVHVAHKVGAESIVLAGGAQAVAAMAYGTESITKVDKILGPGNQFV
                     TAAKMFVSNDTNAAVGIDMPAGPSEVLVIADKDANPAFVASDLLSQAEHGVDSQVILIA
                     IDLDEEHLQAIEDEVHRQATELPRVQIVRGSIAHSITVQVKTVEEAMELSNKYAPEHLI
                     LQIKEAEKAVDLVMNAGSVFIGAWTPESVGDYSAGVNHSLPTYGFGKQYSGVNFASFVK
                     HITSSNLTAEGLKNVGQAVMQLAKVEELEAHRRAVSIRLEHMSKSN"
     promoter        2663..2681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2714..2730
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2887..3074)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(3085..3648)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     promoter        complement(3655..3998)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(4037..4053)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4083..4101)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5460..5471)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(5746..5764)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6022..6610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6784..7641)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7642..7746)
                     /label=AmpR promoter
     promoter        8092..8108
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"