LOCUS       62056_21185        8108 bp DNA     circular SYN 12-OCT-2020
DEFINITION  Cloning vector pTH1150.11, complete sequence.
ACCESSION   MN872812
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Hammond TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-DEC-2019) School of Biological Sciences, Illinois 
            State University, School of Biological Sciences, Normal, IL 61790, 
            USA
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-DEC-2019) School of Biological Sciences, Illinois State 
            University, School of Biological Sciences, Normal, IL 61790, USA"
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(16..1864,1928..2124)
                     /codon_start=1
                     /transl_table=11
                     /product="HIS-3"
                     /label=HIS-3
                     /note="enzyme in histidine biosynthetic pathway"
                     /protein_id="QOI16730.1"
                     /translation="KLAISTILSSVWKSDRPDGLLPTVVVDEHDTALGLVYSSAESVNE
                     ALRTQTGVYQSRKRGLWYKGATSGDTQELVRISLDCDNDALKFVVKQKGRFCHLDQSGC
                     FGQLKGLPKLEQTLISRKQSAPEGSYTARLFSDEKLVRAKIMEEAEELCTAQTPQEIAF
                     EAADLFYFALTRAVAAGVTLADIERSLDAKSWKVKRRTGDAKGKWAEKEGIKPAASALA
                     ATSAPVTKEAAQETTPEKITMRRFDASKVSTEELDAALKRPAQKSSDAIYKIIVPIIED
                     VRKNGDKAVLSYTHKFEKATSLTSPVLKAPFPKELMQLPEETIAAIDVSFENIRKFHAA
                     QKEEKPLQVETMPGVVCSRFSRPIEAVGCYIPGGTAVLPSTALMLGVPAMVAGCNKIVF
                     ASPPRADGTITPEIVHVAHKVGAESIVLAGGAQAVAAMAYGTESITKVDKILGPGNQFV
                     TAAKMFVSNDTNAAVGIDMPAGPSEVLVIADKDANPAFVASDLLSQAEHGVDSQVILIA
                     IDLDEEHLQAIEDEVHRQATELPRVQIVRGSIAHSITVQVKTVEEAMELSNKYAPEHLI
                     LQIKEAEKAVDLVMNAGSVFIGAWTPESVGDYSAGVNHSLPTYGFGKQYSGVNFASFVK
                     HITSSNLTAEGLKNVGQAVMQLAKVEELEAHRRAVSIRLEHMSKSN"
     promoter        2663..2681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2714..2730
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2887..3074)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(3085..3648)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     promoter        complement(3655..3998)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(4037..4053)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4083..4101)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5460..5471)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(5746..5764)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6022..6610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6784..7641)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7642..7746)
                     /label=AmpR promoter
     promoter        8092..8108
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaactcgagc agctgaagct tgccatctcc accatcctct cgagcgtctg gaagtctgac
       61 cgtcccgatg gtcttctccc caccgttgtc gttgatgagc acgacactgc tctgggtctg
      121 gtctacagca gtgccgagag tgtgaacgag gccctcagga cacagactgg tgtctatcag
      181 agccggaagc gcggtctctg gtacaagggt gctacttccg gagacactca ggagctcgtc
      241 cgcatctcgc ttgactgcga taacgatgct ctcaagtttg tcgtgaagca gaagggtcgt
      301 ttctgccacc tcgatcagtc cggctgcttt ggtcagctca aaggccttcc caagctcgag
      361 cagactttga tttcgaggaa acagtctgcc cccgagggct cctacactgc ccgtctcttc
      421 tccgatgaga agctagtccg ggccaagatc atggaggagg ctgaggagct ctgcaccgct
      481 cagacccccc aggaaatcgc ctttgaggct gccgatctct tctactttgc tcttaccagg
      541 gccgttgctg ccggcgttac tcttgccgat atcgaaagga gccttgacgc caagagctgg
      601 aaggtcaagc gcaggactgg agatgctaag ggtaagtggg ctgagaagga gggcatcaag
      661 cctgcggcgt ccgctctcgc tgccacttcg gcccctgtca ccaaggaggc cgcccaggag
      721 accacccctg agaagatcac catgagacgt ttcgacgcct ccaaggtctc taccgaggag
      781 ctcgatgctg ctctcaagcg tcctgcgcaa aagtcgtccg atgccatcta caagatcatt
      841 gtccccatca tcgaggacgt ccgcaagaac ggcgacaagg ctgttctgtc gtacactcac
      901 aagttcgaga aggctacctc tcttactagc cccgtcctga aggcgccctt ccccaaggag
      961 cttatgcagc tccctgagga gaccattgct gccatcgacg tgtccttcga gaacatccgc
     1021 aagttccacg ccgcccagaa ggaggagaag cccctccagg tcgagaccat gcccggtgtt
     1081 gtctgcagcc gtttctctcg tcccatcgag gccgtcggct gctacatccc cggcggtacc
     1141 gccgttctcc ccagcactgc ccttatgctg ggtgttcccg ccatggtcgc cggctgcaac
     1201 aagattgtgt tcgcctctcc tccccgcgcc gacggaacca tcactcccga gattgtccac
     1261 gtcgctcaca aggttggggc cgagtccatc gtgcttgccg gcggtgccca ggccgtagct
     1321 gccatggcct acggcaccga gagcatcacc aaggtcgaca agattctcgg ccccggtaac
     1381 cagttcgtca ctgctgccaa gatgttcgtc agcaacgaca ccaacgctgc cgttggtatt
     1441 gacatgcccg ctggcccgtc cgaggtgctg gtcatcgctg acaaggacgc caaccccgcg
     1501 ttcgttgcct cggatctcct gtcccaggct gagcacggcg ttgacagtca ggtcatcctg
     1561 atcgctattg acctcgacga ggagcatctt caggctattg aggacgaggt tcaccgtcag
     1621 gctacggagc ttcctcgcgt ccagattgtc cgtggctcca tcgcccactc gatcaccgtg
     1681 caggtcaaga ccgtcgagga ggccatggag ctcagcaaca agtacgctcc tgagcacttg
     1741 atcctccaga tcaaggaggc cgagaaggct gtcgatcttg tcatgaacgc cggtagtgtc
     1801 ttcattggcg cctggactcc tgagtccgtt ggcgattact ctgctggtgt taaccactcg
     1861 ctgcgtaagt tacatatcat aaatagcccc gcttcacaga ttcttctgct aacgtcaaga
     1921 cacatagcta cctatggctt tggcaagcag tactctggcg tcaatttcgc ctcgttcgtc
     1981 aagcacatta ccagctccaa cttgactgcc gagggtctca aaaacgtcgg ccaggctgtc
     2041 atgcagttgg ctaaggttga ggagctcgag gctcacagaa gggcggtcag catccgtctt
     2101 gagcacatga gcaagagcaa ctaaacggaa attcttttcg aagtagcaaa aaaaaaaaaa
     2161 aaaaacaaga acaaaaggat gtagtgggtt gatgtatatc tgggtcattt tgggcacata
     2221 gagtaatgat aacgagtttt ggacattgta ctgttctgta caggctgaag atcagtacat
     2281 gaatctgttg gtaagtgtgg agacccaaac ggcccttgag tttttctccc tattccagca
     2341 gatgaggtgc tcgtccctgg gtgtttattt tcattattac atcaaccttt tttttttttt
     2401 tttttttcag atcatgcgta catgaacggg ggaagcacag acgatcgaaa cgtggatgtc
     2461 acaatgtccc tgcagtgatg ctgcattgca tgaagcgccc atctcaaaaa acttgcagtc
     2521 ttgcacgttg catgtgaacc tcccaaacaa ccgaataaaa gacggcgaaa aatgaagata
     2581 aaaaaaaacc ataaaaaaaa tcagagggag tgtgggaaat ggtgtctttt agcattcaga
     2641 ccccattagc cgtccacgcc cttaatacga ctcactatag ggcgaattgg agctccaccg
     2701 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg cccttaacat
     2761 atgccgcaca ggtgttgtcc tctgaggaca taaaatacac accgagattc atcaactcat
     2821 tgctggagtt agcatatcta caattgggtg aaatggggag cgatttgcag gcatttgctc
     2881 ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat gctatacctg agaaagcaac
     2941 ctgacctaca ggaaagagtt actcaagaac aagaattttc gttttaaaac ctaagagtca
     3001 ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa aatcataaat
     3061 cataagaaat tcgcctcgag attaggggca gggcatgctc atgtagagcg cctgctcgcc
     3121 gtccgaggcg gtgccgtcgt acagggcggt gtccaggccg cagagggtga accccatccg
     3181 ccggtacgcg tggatcgccg gtgcgttgac gttggtgacc tccagccaga ggtgcccggc
     3241 gccccgctcg cgggcgaact ccgtcgcgag ccccatcaac gcgcgcccga ccccgtgccc
     3301 ccggtgctcc ggggcgacct cgatgtcctc gacggtcagc cggcggttcc agccggagta
     3361 cgagacgacc acgaagcccg ccaggtcgcc gtcgtccccg tacgcgacga acgtccggga
     3421 gtccgggtcg ccgtcctccc cgtcgtccga ttcgtcgtcc gattcgtcgt cggggaacac
     3481 cttggtcagg ggcgggtcca ccggcacctc ccgcagggtg aagccgtccc cggtggcggt
     3541 gacgcggaag acggtgtcgg tggtgaagga cccatccagt gcctcgatgg cctcggcgtc
     3601 ccccgggaca ctggtgcggt accggtaagc cgtgtcgtca agagtggtac ccatggttgt
     3661 ttatgttcgg atgtgatgtg agaactgtat cctagcaaga ttttaaaagg aagtatatga
     3721 aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg cgcggcgtgg
     3781 ggacaattca acgcgtctgt gaggggagcg tttccctgct cgcaggtctg cagcgaggag
     3841 ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa tgattataca
     3901 tggggatgta tgggctaaat gtacgggcga cagtcacatc atgcccctga gctgcgcacg
     3961 tcaagactgt caaggagggt attctgggcc tccatgtcgc tcatatgtta agggcgaatt
     4021 ccgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt
     4081 tccctttagt gagggttaat tagggccaag ctaccccgtc aaatctatac atatctataa
     4141 cgggtttcag gggtttcgtt cgctgtcaat caagtttgaa acatcactgg ggccgttgga
     4201 cggtgtatta gaccattggc tccctcagct gtttggcggc tgggcggctg ggtcaaacgg
     4261 caataacggg actcgagagg gacgaggaga gtcggttggc tggctgcaat acaagcgttc
     4321 ccacctaacc aacgagtccc gttttccatt tgtgtgccta accatcatct agggatgtca
     4381 gggtttggcc ggatcagggt atgtttggtt gactgttgtc atgtctgatt gggtacatat
     4441 tatggtaggt gtctcgagaa cagtagagta ctcgggccta gcgtttggat gattacgcga
     4501 gatatgagtt gtgggccgcc atgcagttgc ttgtccataa gcagaagttg ctttgggata
     4561 tatttctcgt ctttcaaagg tcacgaggtc ctgggacgaa cggcatcgcc atccaaaggg
     4621 ttgaacatga gaaacctgaa tggcctttgc gttgaaatac aaaaagtcaa gaacaaaatc
     4681 gcttgaggat agggacgtgg aagcaagcaa atatggtaag agaggtatac atcaaccctg
     4741 gttcaattgt tagcgtggtt cttcctccac gtcctcgttc atgacggtta acagtaccag
     4801 gctaacaatt aaaccagggt tgatgtgtac tgatatgtag gtgctcagca aactgccaat
     4861 ttcttggccc caagcagcag tttgctgtca gtgctgctcg tgtcagcctt ggtagtggaa
     4921 cctaaactgc taacacagcg caagtgcgca tgtaaagata ttgtgggagg atctgtatgg
     4981 atggatgaga ttactgcttg gtgttggttg cgaggcactg cggctgttag gctttgctgt
     5041 gccccgttcg acgaagaaat acgcggaact ataaattgga tacctagact tactgcctat
     5101 gggaggtatc taccgacgta gccgacggat tctagcaaca tcccgacttt gcttgtagtg
     5161 tactatgata gcagcacagt gttgctcctt gtgagaatgg gctctttttt tttttccccc
     5221 ttccctaggg cgttgactgg acttgctcta ttgttcccaa ggtaggtgcc cgtcatcgat
     5281 tttcccaagt ctcccgccag attgtcgtca tagtgtcatg atgacctcgg tcgctggggc
     5341 tgcgtggtta cggggagctg ggaccgctag gcctacagtg gttgtgccat tcagcgtggg
     5401 tgtgtggagt agcggtagag gcgcttggaa gttgtgctag cggaaaccct ggaatatctt
     5461 ctaccctcga ttccttctcg ggctgcccat gtgctgaggt gatgccgggg atctggcgcc
     5521 aatcatccat tgaggttccc gcagcttccc ggtgccgcgc gcgggcgcag ttgctcacag
     5581 gacacaccta gacgcagggg cacaggggca ccgtttggtg tgcaactggg tacctagctg
     5641 tagcaagcac tccaccgtct gtgcaatccc ccaatccacg gcaggaactt cgcaccgccg
     5701 cggcaccgag tgagcgaatc catccgcatt ggatctgccg gtctccctat agtgagtcgt
     5761 attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg
     5821 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6181 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     6241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6421 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7081 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7381 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7741 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7801 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     7861 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     7921 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     7981 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc
     8041 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     8101 acactata
//
