pBOB11_C-Term vector (V016022)

LOCUS       62056_4155        8509 bp DNA     circular SYN 05-FEB-2020
DEFINITION  Cloning vector pBOB11_C-Term, complete sequence.
ACCESSION   MN991175
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Alvarez JM, Schinke A-L., Brooks MD, Pasquino AA, Leonelli L, Varala
            K, Safi A, Krouk G, Krapp A, Coruzzi GM.
  TITLE     Transient genome-wide interactions of the master transcription 
            factor NLP7 initiate a rapid nitrogen-response cascade
  JOURNAL   Nat Commun (2020) In press
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Doidy J, Coruzzi GM.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2020) Biology - Center for Genomics and Systems 
            Biology, New York University, 12 Waverly Place, New York, NY 10003, 
            USA
REFERENCE   3  (bases 1 to 8509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2020) Biology - Center for Genomics and Systems Biology, New
            York University, 12 Waverly Place, New York, NY 10003, USA"
FEATURES             Location/Qualifiers
     source          1..8509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      126..581
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        659..763
                     /label=AmpR promoter
     CDS             764..1621
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1795..2383
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2671..2692
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2707..2737
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2745..2761
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2769..2785
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2803..2821
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        3157..3502
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3569..3592
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             3599..4273
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      4308..4560
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5199..5543
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5648..5772
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5797..5827
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5881..6537
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             6882..7184
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(7228..7352)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7354..8190
                     /note="Region: C-terminal glucocorticoid receptor; GR"
     promoter        complement(8453..8471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8478..8494)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"