LOCUS       62056_4155        8509 bp DNA     circular SYN 05-FEB-2020
DEFINITION  Cloning vector pBOB11_C-Term, complete sequence.
ACCESSION   MN991175
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Alvarez JM, Schinke A-L., Brooks MD, Pasquino AA, Leonelli L, Varala
            K, Safi A, Krouk G, Krapp A, Coruzzi GM.
  TITLE     Transient genome-wide interactions of the master transcription 
            factor NLP7 initiate a rapid nitrogen-response cascade
  JOURNAL   Nat Commun (2020) In press
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Doidy J, Coruzzi GM.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2020) Biology - Center for Genomics and Systems 
            Biology, New York University, 12 Waverly Place, New York, NY 10003, 
            USA
REFERENCE   3  (bases 1 to 8509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2020) Biology - Center for Genomics and Systems Biology, New
            York University, 12 Waverly Place, New York, NY 10003, USA"
FEATURES             Location/Qualifiers
     source          1..8509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      126..581
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        659..763
                     /label=AmpR promoter
     CDS             764..1621
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1795..2383
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2671..2692
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2707..2737
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2745..2761
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2769..2785
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2803..2821
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        3157..3502
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3569..3592
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             3599..4273
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      4308..4560
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5199..5543
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5648..5772
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5797..5827
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5881..6537
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             6882..7184
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(7228..7352)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7354..8190
                     /note="Region: C-terminal glucocorticoid receptor; GR"
     promoter        complement(8453..8471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8478..8494)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
       61 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      121 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      541 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
      601 cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg
      661 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      721 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      781 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      841 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      901 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      961 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1021 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1081 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1141 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1201 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1261 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1321 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1381 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1441 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1501 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1561 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1621 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1681 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1741 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1801 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1861 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1921 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1981 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2041 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2101 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2161 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2221 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2281 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2341 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2401 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2461 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2521 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2581 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2641 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2701 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2761 atttcacaca ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag
     2821 aatactcaag ctatgcatcc aacgcgttgg gagctctccc atatgggaga gctcaagctt
     2881 ctgcaggtcc gatgtgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     2941 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     3001 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     3061 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     3121 tcttcaaagc aagtggattg atgtgatggt ccgatgtgag acttttcaac aaagggtaat
     3181 atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt
     3241 ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga
     3301 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     3361 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     3421 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     3481 ttcatttcat ttggagagga cacgctgaca agctgactct agcagatcta tcgattctag
     3541 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccgat
     3601 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
     3661 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3721 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     3781 gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3841 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3901 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3961 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     4021 gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     4081 caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta
     4141 catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat
     4201 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca
     4261 ctccaccggc gcctaagaat tcgagctcgg taccggatcc aattcccgat cgttcaaaca
     4321 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4381 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4441 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4501 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     4561 ggggatcgat ccccgggcat atggtcgact agagccaagc tgatctcctt tgccccggag
     4621 atcaccatgg acgactttct ctatctctac gatctaggaa gaaagttcga cggagaaggt
     4681 gacgatacca tgttcaccac cgataatgag aagattagcc tcttcaattt cagaaagaat
     4741 gctgacccac agatggttag agaggcctac gcggcaggtc tcatcaagac gatctacccg
     4801 agtaataatc tccaggagat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
     4861 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
     4921 attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat agagattgga
     4981 gtctctaaga aagtagttcc tactgaatca aaggccatgg agtcaaaaat tcagatcgag
     5041 gatctaacag aactcgccgt gaagactggc gaacagttca tacagagtct tttacgactc
     5101 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctcgt ctactccaag
     5161 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     5221 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca
     5281 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
     5341 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     5401 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     5461 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     5521 agttcatttc atttggagag gactccggta tttttacaac aataccacaa caaaacaaac
     5581 aacaaacaac attacaattt actattctag tcgacctgca ggcggccgca ctagtactag
     5641 tgatatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     5701 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     5761 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
     5821 tataatgtgt ggattttgag ttaggatccg gcgagatttt caggagctaa ggaagctaaa
     5881 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     5941 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     6001 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     6061 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     6121 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     6181 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     6241 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     6301 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     6361 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     6421 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat
     6481 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     6541 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     6601 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta
     6661 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     6721 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     6781 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     6841 aatgaacggc tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca
     6901 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     6961 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     7021 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     7081 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     7141 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     7201 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
     7261 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     7321 gtttctcgtt cagctttctt gtacaaagtg gatgaaattc agcaagccac tgcaggagtc
     7381 tcacaagaca cttcggaaaa tcctaacaaa acaatagttc ctgcagcatt accacagctc
     7441 acccctacct tggtgtcact gctggaggtg attgaacccg aggtgttgta tgcaggatat
     7501 gatagctctg ttccagattc agcatggaga attatgacca cactcaacat gttaggtggg
     7561 cgtcaagtga ttgcagcagt gaaatgggca aaggcgatac taggcttgag aaacttacac
     7621 ctcgatgacc aaatgaccct gctacagtac tcatggatgt ttctcatggc atttgccttg
     7681 ggttggagat catacagaca atcaagcgga aacctgctct gctttgctcc tgatctgatt
     7741 attaatgagc agagaatgtc tctaccctgc atgtatgacc aatgtaaaca catgctgttt
     7801 gtctcctctg aattacaaag attgcaggta tcctatgaag agtatctctg tatgaaaacc
     7861 ttactgcttc tctcctcagt tcctaaggaa ggtctgaaga gccaagagtt atttgatgag
     7921 attcgaatga cttatatcaa agagctagga aaagccatcg tcaaaaggga agggaactcc
     7981 agtcagaact ggcaacggtt ttaccaactg acaaagcttc tggactccat gcatgaggtg
     8041 gttgagaatc tccttaccta ctgcttccag acatttttgg ataagaccat gagtattgaa
     8101 ttcccagaga tgttagctga aatcatcact aatcagatac caaaatattc aaatggaaat
     8161 atcaaaaagc ttctgtttca tcaaaaatga tatcccgcgg ccatgctaga gtccgcaaaa
     8221 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     8281 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     8341 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     8401 ctaaaaccaa aatccagtga cctgcaggca tgcgacgtcg ggcccaattc gccctatagt
     8461 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggg
//
