p5L-R vector (V015981)

LOCUS       V015981                 6383 bp    DNA     circular SYN 09-DEC-2019
DEFINITION  Exported.
ACCESSION   V015981
VERSION     V015981
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Lyu Z, Shao N, Chou CW, Shi H, Patel R, Duin EC, Whitman WB.
  TITLE     Post-translational methylation of arginine in methyl-coenzyme M
            reductase has a profound impact on both methanogenesis and growth of
            Methanococcus maripaludis
  JOURNAL   J. Bacteriol. (2019) In press
   PUBMED   31740491
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Lyu Z, Whitman WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Molecular Virology and Microbiology, Baylor
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 6383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-AUG-2019) Molecular Virology and Microbiology, Baylor College of
            Medicine, One Baylor Plaza, Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..6383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(192..1133)
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /label="nitrogenase iron protein"
                     /note="MMP0147; MMP_RS00840"
                     /protein_id="QGR24485.1"
                     /translation="MYYIVLLILRESFIFMLNGDILKQIAFYGKGGIGKSTTVCNLAAA
                     LSKSGKKVIVVGCDPKHDCTSNLRSGEDIPTVLDVLREKGIDKLGIETIIRENLLKKED
                     IIYEGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKKMNVFEDLDVDVVLYDVLGDVVC
                     GGFAMPLRMGLADQIYVVTSSDYMALYAANNICSGISQFVKRGGSTLGGIIYNVRGSMD
                     AFDIVSEFANQLNANIIGKVPNSPIINEAEIDGQTAIEYAPEEEISKIYMELAESIYQN
                     NTGTMPNPLENAQIMQIGKMIKERIKKQKITE"
     primer_bind     1550..1566
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      1588..1761
                     /label="PhmvA"
                     /note="PhmvA"
                     /regulatory_class="promoter"
     regulatory      1771..1776
                     /label="Mv_hmv"
                     /note="Mv_hmv"
                     /regulatory_class="ribosome_binding_site"
     CDS             1782..2336
                     /gene="hpt"
                     /label="Hypoxanthine/guanine phosphoribosyltransferase"
                     /note="Hypoxanthine/guanine phosphoribosyltransferase from
                     Methanococcus maripaludis (strain S2 / LL). Accession#:
                     Q6M0X3"
     CDS             2360..2956
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     regulatory      2989..3014
                     /label="Tmcr"
                     /note="Tmcr"
                     /regulatory_class="terminator"
     repeat_region   3021..3080
                     /rpt_family="RE60"
     misc_feature    complement(3081..4073)
                     /note="similar to SAM-dependent methyltransferase; MMP0149;
                     MMP_RS00850"
     primer_bind     complement(4162..4178)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4186..4202)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4210..4240)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4255..4276)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4564..5152)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5326..6183)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6184..6288)
                     /label="AmpR promoter"