LOCUS       V015981                 6383 bp    DNA     circular SYN 09-DEC-2019
DEFINITION  Exported.
ACCESSION   V015981
VERSION     V015981
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Lyu Z, Shao N, Chou CW, Shi H, Patel R, Duin EC, Whitman WB.
  TITLE     Post-translational methylation of arginine in methyl-coenzyme M
            reductase has a profound impact on both methanogenesis and growth of
            Methanococcus maripaludis
  JOURNAL   J. Bacteriol. (2019) In press
   PUBMED   31740491
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Lyu Z, Whitman WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Molecular Virology and Microbiology, Baylor
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 6383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-AUG-2019) Molecular Virology and Microbiology, Baylor College of
            Medicine, One Baylor Plaza, Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..6383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(192..1133)
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /label="nitrogenase iron protein"
                     /note="MMP0147; MMP_RS00840"
                     /protein_id="QGR24485.1"
                     /translation="MYYIVLLILRESFIFMLNGDILKQIAFYGKGGIGKSTTVCNLAAA
                     LSKSGKKVIVVGCDPKHDCTSNLRSGEDIPTVLDVLREKGIDKLGIETIIRENLLKKED
                     IIYEGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKKMNVFEDLDVDVVLYDVLGDVVC
                     GGFAMPLRMGLADQIYVVTSSDYMALYAANNICSGISQFVKRGGSTLGGIIYNVRGSMD
                     AFDIVSEFANQLNANIIGKVPNSPIINEAEIDGQTAIEYAPEEEISKIYMELAESIYQN
                     NTGTMPNPLENAQIMQIGKMIKERIKKQKITE"
     primer_bind     1550..1566
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      1588..1761
                     /label="PhmvA"
                     /note="PhmvA"
                     /regulatory_class="promoter"
     regulatory      1771..1776
                     /label="Mv_hmv"
                     /note="Mv_hmv"
                     /regulatory_class="ribosome_binding_site"
     CDS             1782..2336
                     /gene="hpt"
                     /label="Hypoxanthine/guanine phosphoribosyltransferase"
                     /note="Hypoxanthine/guanine phosphoribosyltransferase from
                     Methanococcus maripaludis (strain S2 / LL). Accession#:
                     Q6M0X3"
     CDS             2360..2956
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     regulatory      2989..3014
                     /label="Tmcr"
                     /note="Tmcr"
                     /regulatory_class="terminator"
     repeat_region   3021..3080
                     /rpt_family="RE60"
     misc_feature    complement(3081..4073)
                     /note="similar to SAM-dependent methyltransferase; MMP0149;
                     MMP_RS00850"
     primer_bind     complement(4162..4178)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4186..4202)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4210..4240)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4255..4276)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4564..5152)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5326..6183)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6184..6288)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgta tctattcagt aattttctgt ttttttattc tttctttaat catttttcca
      241 atctgcatta tttgggcgtt ttccaatgga ttgggcatgg ttccagtatt attttggtat
      301 atactttctg caagttccat atatatttta cttatttctt cttctggagc atattctatt
      361 gcggtctgtc catcaatttc agcttcattg attattgggg aatttggaac ttttccaata
      421 atgtttgcat ttaactggtt tgcaaattca cttacaatat caaatgcgtc cattgaaccc
      481 cttacgttgt aaattatacc tccaagggta cttcctccac gttttacaaa ctggcttatt
      541 ccactgcata tattatttgc agcatagagc gccatgtaat ctgaagatgt tacaacgtaa
      601 atctggtcag caagacccat tctaagcggc attgcaaatc ctccgcacac aacatcgcct
      661 aagacatcgt aaagtactac atcaacatct aggtcttcaa aaacgttcat ttttttgagg
      721 aggtctatta caacaatcac ccctcttcct gcacatccat atcctggttt tgggccgcct
      781 gcttcgacgc agtaaatccc gttaaatcct tcataaatta tatcttcttt ttttagtaaa
      841 ttttcgcgaa ttatagtttc aatcccgagt ttatcgattc ccttttccct caaaacatct
      901 aaaacagtag gaatatcttc tccacttctt aaattagatg tacaatcgtg ttttgggtcg
      961 catccaacaa cgataacttt tttaccactt ttggatagcg cagctgctaa attacaaaca
     1021 gtagtggact ttccaatgcc tcctttcccg taaaatgcaa tctgtttcaa aatatcaccg
     1081 tttaacatga atatgaagga ttctcgtaaa attaataaaa caatatagta catataaccg
     1141 gaataactaa tataatttat tattaataaa ctattgattg aaaaatcacg ataatgctgt
     1201 ttttatttaa taacccttat ttttaaaaaa ctgataatta actgaattaa aaaaataatt
     1261 cctattttat tcagcaatat ttcgttactt gtcggattta ataattcctc tgtagaaatt
     1321 tatatatact ttgtcgtatc tataatgatt aataatgaca aataaaggta ccgcacagat
     1381 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     1441 aagggcgatc ggtgcgggcc tcttcgctat tacgccctta aggcgaaagg gggatgtgct
     1501 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     1561 gccagtgaat tcgcgcccgg ttctagacga gataagaatt actagataat tcagctgatc
     1621 gatcaaaata taacataaat aacataggtt taaataattt aaaggcatat tttatataaa
     1681 caattgtaaa atattggctt atgaaatttg ttaaaattta gctaataagc tattgatatt
     1741 ataattttat agataactaa tactagaaat aggtgaaatg catgagcaaa ttattggaag
     1801 aatcattgaa aacatgccct attgttaaaa gaggagaata ccactacttt atacacccga
     1861 tatctgatgg agttcccctt gttgaaccag aattattgag agatgtttca acaagagtta
     1921 tcaaaatgat agatacggat gttgacaaaa tagtgacagc agaagccatg ggaataccaa
     1981 ttgtaactgc ggtatcgatt gcaacagata tcccgtatgt tataatgaga aaaagagagt
     2041 acttgctcga aggggaaatt ccagttcatc aagaaactgg atacagtaaa ggagaacttt
     2101 acttaaacgg gataaataaa ggggacaaag ttataatcct tgatgacgtt atatcaacag
     2161 gtggaaccct tgttgcaatt ataaacgcgt taaaaagagc tggtgcagat atcaaagatg
     2221 ttttgtgcat aattgacaga ggaaacggtc aaaacattgt tgaagaaaaa accggataca
     2281 aagtaaaaac tattgtaaaa attgaagttg ttgacggaaa agtaaacatt ttagaataaa
     2341 ctagaggaga cgaccttcca tgacagaata taaaccaaca gttagattag caacaagaga
     2401 tgacgtacct agggcagtaa gaactcttgc agcagctttt gcagattatc cagcaacaag
     2461 acacacagtt gatccagata gacacattga aagagttaca gaattacaag aattattttt
     2521 aacaagagtt ggtcttgata taggtaaagt atgggttgca gatgatggag cagcagttgc
     2581 agtttggaca acaccagaat cagttgaagc tggtgctgtt tttgcagaaa ttggaccaag
     2641 aatggctgaa ttatcaggat caagattagc agctcagcaa cagatggaag gattacttgc
     2701 accacacaga ccaaaagaac cagcatggtt tttagcaaca gttggagttt caccagatca
     2761 ccagggaaaa ggtttaggtt ctgctgttgt acttcctggt gttgaagcag ctgaaagagc
     2821 aggagttcca gcatttttag aaacatcagc accaagaaat cttccatttt atgaaagact
     2881 tggatttaca gttacagcag atgttgaagt tccagaagga ccaagaacat ggtgtatgac
     2941 aagaaaacca ggagcataat ttagtaaata atatggttta aaaaaattaa aggaaaccta
     3001 atatggtttc ctttactagt cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     3061 gttgtaaaac gacggccagt gaattccact aaatttaaat caagttccat tttatttggt
     3121 gctgcaaata ctaaataatc attatctttt aaaacatcta caattttttc aaatatttct
     3181 aacatgtccc cttttttaga tgtggaaatt ccatatggtg gatcagttac aattccatct
     3241 atcttttcaa ttttcagggc ttccaaatat tttgagacgt ttttagcatc gttttctttt
     3301 attgaaatga cttgttttga taaatcatat gtgtttaagt ttagtagtgc accatttacc
     3361 atctgttcat cgatgtcact accaattaat ttgcatccta aaaaacccgc ttcaattaag
     3421 aatcctcctg ttccgcagaa tggatctaaa acaatttccc cttctttaac tcttgataga
     3481 tttacaaggc atcttgcaag ttttggcatt atgcatcctg gatgaaaata tgctcttaag
     3541 tggggcctgt tattttggaa gtattcaaca tctcgttttt caataactat tccggtatac
     3601 attttatcgt ttgaaataac gactttaacc gtttttgaag ggttttttaa acttacctgt
     3661 gcagaagttt ttttctttat tatccctccg agctttcttt cgatttccat cgaattaaac
     3721 gggctttctt tttccaattt taaaacacga actgcaaaag tttcatctct ttcaactgga
     3781 aaatcgagtg tttcagaact tttaaaaaaa ttttcaagtg tttcatccac attctggtta
     3841 attattgggg attctgaaaa tataacgtgt ccttcgtttg tgtatcctcc ccttttaacc
     3901 atttttttta taatttcttc tgaaattgaa ctgtccaata ttacgtaatt tttaaagcag
     3961 tttaaattac cttcaagact aaaaatatcg attaaagcat ttaattctgc aatcggtagt
     4021 ttttcgtgtt ctgcacttaa caaaaaacct gcttttgtgt tttttaaatt caacatatca
     4081 ccagtaggtt taaatttcaa aacatcactg cagttttata tatttttaat tcactggggg
     4141 gataagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     6361 gcgtatcacg aggccctttc gtc
//
