pJ01 vector (V015931)

LOCUS       62056_13035        2541 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pJ01, complete sequence.
ACCESSION   MK234843
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(83..126)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        144..178
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     regulatory      179..190
                     /label=B0034
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             203..232
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             248..265
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      491..562
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      578..605
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(810..1398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1572..2429)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2430..2534)
                     /label=AmpR promoter