LOCUS       62056_13035        2541 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pJ01, complete sequence.
ACCESSION   MK234843
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(83..126)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        144..178
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     regulatory      179..190
                     /label=B0034
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             203..232
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             248..265
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      491..562
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      578..605
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(810..1398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1572..2429)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2430..2534)
                     /label=AmpR promoter
ORIGIN
        1 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
       61 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
      121 gatttctgga attctaaaga tctttgacag ctagctcagt cctaggtata atactagtaa
      181 agaggagaaa aagcttgggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc
      241 cgtcgaccat catcatcatc atcattgagt ttaaacggtc tccagcttgg ctgttttggc
      301 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
      361 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
      421 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
      481 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      541 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      601 ctgcgtggat ccttactcga gtctagactg caggcttcct cgctcactga ctcgctgcgc
      661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1561 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1621 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1681 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     1741 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1801 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     1861 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     1921 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     1981 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2041 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2101 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2161 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2221 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2281 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2341 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2401 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2461 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2521 ataggggttc cgcgcacatt t
//
