Basic Vector Information
- Vector Name:
- pNC-BiFC-ED1
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 9884 bp
- Type:
- Plant binary expression vector
- Replication origin:
- pSa ori
- Host:
- Plants
- Source/Author:
- Yan P, Zeng Y, Shen W, Tuo D
- Promoter:
- CaMV 35S (enhanced)
pNC-BiFC-ED1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNC-BiFC-ED1 vector Sequence
LOCUS 62056_17760 9884 bp DNA circular SYN 04-SEP-2019
DEFINITION Plant binary expression vector pNC-BiFC-ED1, complete sequence.
ACCESSION MK896890
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9884)
AUTHORS Yan P, Zeng Y, Shen W, Tuo D, Li X, Zhou P.
TITLE Direct Submission
JOURNAL Submitted (07-MAY-2019) Institute of Tropical Bioscience and
Biotechnology, Chinese Academy of Tropical Agriculture Sciences,
Xueyuan Road, Haikou, Hainan 571101, China
REFERENCE 2 (bases 1 to 9884)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(07-MAY-2019) Institute of Tropical Bioscience and Biotechnology,
Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road,
Haikou, Hainan 571101, China"
FEATURES Location/Qualifiers
source 1..9884
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 10..26
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 118..789
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
regulatory 799..929
/label=translational enhancer
/note="translational enhancer"
/regulatory_class="enhancer"
CDS 932..1450
/label=VN173
/note="N-terminal fragment of mVenus for use in bimolecular
fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
promoter 1650..1752
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 1783..2085
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
regulatory 2155..2365
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
CDS complement(2475..3128)
/label=CmR
/note="chloramphenicol acetyltransferase"
regulatory 3227..3437
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
CDS complement(3507..3809)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
promoter complement(3840..3942)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
gene 4139..4333
/gene="CEYFP"
/label=CEYFP
regulatory 4342..4472
/label=translational enhancer
/note="translational enhancer"
/regulatory_class="enhancer"
promoter complement(4482..5153)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind complement(5245..5261)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5284..5300)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(5337..5355)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5376..5392)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5400..5416)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5424..5455)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5470..5491)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5730..5754
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(5845..6433)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6537..7349)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 7640..8075
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 8208..8230
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
terminator complement(8254..8505)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(8548..9339)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(9373..9552)
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 9822..9840
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 9866..9882
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
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