LOCUS       62056_17760        9884 bp DNA     circular SYN 04-SEP-2019
DEFINITION  Plant binary expression vector pNC-BiFC-ED1, complete sequence.
ACCESSION   MK896890
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Yan P, Zeng Y, Shen W, Tuo D, Li X, Zhou P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2019) Institute of Tropical Bioscience and 
            Biotechnology, Chinese Academy of Tropical Agriculture Sciences, 
            Xueyuan Road, Haikou, Hainan 571101, China
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAY-2019) Institute of Tropical Bioscience and Biotechnology, 
            Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road, 
            Haikou, Hainan 571101, China"
FEATURES             Location/Qualifiers
     source          1..9884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        118..789
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      799..929
                     /label=translational enhancer
                     /note="translational enhancer"
                     /regulatory_class="enhancer"
     CDS             932..1450
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     promoter        1650..1752
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1783..2085
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      2155..2365
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     CDS             complement(2475..3128)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      3227..3437
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     CDS             complement(3507..3809)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     promoter        complement(3840..3942)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     gene            4139..4333
                     /gene="CEYFP"
                     /label=CEYFP
     regulatory      4342..4472
                     /label=translational enhancer
                     /note="translational enhancer"
                     /regulatory_class="enhancer"
     promoter        complement(4482..5153)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     complement(5245..5261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5284..5300)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5337..5355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5376..5392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5400..5416)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5424..5455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5470..5491)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    5730..5754
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5845..6433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6537..7349)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7640..8075
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    8208..8230
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(8254..8505)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8548..9339)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(9373..9552)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        9822..9840
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9866..9882
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc catggtgagc aagggcgagg agctgttcac
      961 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1021 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1081 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     1141 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1201 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1261 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1321 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1381 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1441 caacatcgag gactccggac tcagatctca gtggtctctg tccagtcctg gcctcgtcgg
     1501 ccattctcga ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     1561 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     1621 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     1681 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1741 aaggaagcta aacttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac
     1801 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1861 acgcctgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1921 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1981 gatatggcca gtgtgccggt atccgttatc ggggaagaag tggctgatct cagccaccgc
     2041 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2101 cttatacaca gggccagtct ggccacttgt ggtctgctga gaccctgatc tagagtccgc
     2161 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     2221 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     2281 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     2341 attcctaaaa ccaaaatcca gtgacgctta tgggaccgac atatcagtat atattcttat
     2401 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     2461 acgcggcgtt tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt
     2521 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     2581 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     2641 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     2701 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2761 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2821 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2881 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
     2941 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     3001 gccgtaatat ccagctgaac ggtctggtta taggtacatt gaacaactga ctgaaatgcc
     3061 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     3121 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     3181 agtgatctta tttcattatg gtgaaagttg gaacctctta ccgggcgtca ctggattttg
     3241 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     3301 ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt cccttatctg
     3361 ggaactactc acacattatt ctggagaaaa atagagagag atagatttgt agagagagac
     3421 tggtgatttt tgcggactct agatcaccac tggaaacatc caggtctggc cagactggcc
     3481 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
     3541 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga taccggcaca
     3601 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
     3661 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
     3721 cgcccaggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
     3781 cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt cagcaaaagt
     3841 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3901 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa
     3961 aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag
     4021 tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta
     4081 gtcgagaatg gccgacgagg ccacgcatgg tcgagccaac tctagatctg agtccggact
     4141 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     4201 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt
     4261 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4321 gctgcacgct gccgcccatg gctatcgttc gtaaatggtg aaaattttca gaaaattgct
     4381 tttgctttaa aagaaatgat ttaaattgct gcaatagaag tagaatgctt gattgcttga
     4441 gattcgtttg ttttgtatat gttgtgttga gaactctcga cgtcctctcc aaatgaaatg
     4501 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
     4561 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
     4621 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
     4681 cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt
     4741 ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg
     4801 atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga
     4861 tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct
     4921 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     4981 ctttgggacc actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat
     5041 ggcatttgta ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg
     5101 ggcaatggaa tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc
     5161 tttggtcttc tgagactgta tctttgatat ttttggagta gacaagtgtg tcgtgctcca
     5221 catgttgacc ggtggcgcgc cggcactggc cgtcgtttta caacgaattc ctgcagcccg
     5281 ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     5341 tagtgagggt taattccgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5401 tgttatccgc tcacaattcc acacaacata cgagccggaa ghcataaagt gtaaagcctg
     5461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5521 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5581 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5701 ggataacgca ggaaagaaca tgaaggcctt gacaggatat attggcgggt aaactaagtc
     5761 gctgtatgtg tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5821 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5881 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5941 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6001 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6061 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6121 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6181 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6241 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6301 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6361 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6421 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6481 aaactcacgt taagggattt tggtcatggt tacaaccaat taaccaattc tgattagaaa
     6541 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     6601 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     6661 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     6721 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     6781 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     6841 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     6901 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     6961 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     7021 aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga
     7081 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     7141 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     7201 ggcgcatcgg gcttcccata caatccatag attgtcgcac ctgattgccc gacattatcg
     7261 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag
     7321 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     7381 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     7441 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     7501 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
     7561 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
     7621 ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggcttt tttatccccg
     7681 gaagcctgtg gatagagggt agttatccac gtgaaaccgc taatgccccg caaagccttg
     7741 attcacgggg ctttccggcc cgctccaaaa actatccacg tgaaatcgct aatcagggta
     7801 cgtgaaatcg ctaatcggag tacgtgaaat cgctaataag gtcacgtgaa atcgctaatc
     7861 aaaaaggcac gtgagaacgc taatagccct ttcagatcaa cagcttgcaa acacccctcg
     7921 ctccggcaag tagttacagc aagtagtatg ttcaattagc ttttcaatta tgaatatata
     7981 tatcaattat tggtcgccct tggcttgtgg acaatgcgct acgcgcaccg gctccgcccg
     8041 tggacaaccg caagcggttg cccaccgtcg agcgccagcg cctttgccca caacccggcg
     8101 gccggccgca acagatcgtt ttataaattt ttttttttga aaaagaaaaa gcccgaaagg
     8161 cggcaacctc tcgggcttct ggatttccga tccccggaat tagatcttgg caggatatat
     8221 tgtggtgtaa cgttatcagc ttgcatgccg gtcgatctag taacatagat gacaccgcgc
     8281 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
     8341 attgcgggac tctaatcaaa aaacccatct cataaataac gtcatgcatt acatgttaat
     8401 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
     8461 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctagct
     8521 agagtccgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     8581 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     8641 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     8701 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     8761 caagcaggca tcgccctggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag
     8821 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     8881 gacaagaccg gcttccatcc gagtacgtcc tcgctcgatg cgatgtttcg cttggtggtc
     8941 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     9001 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     9061 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     9121 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgg agttcattca gggcaccgga
     9181 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     9241 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     9301 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cctcgatcga gttgagagtg
     9361 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     9421 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     9481 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca
     9541 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     9601 cataacgtga ctcccttaat tctcatgtat cgataacatt aacgtttaca atttcgcgcc
     9661 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9721 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9781 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     9841 gcgaattggg taccgggccc cccctcgagg tcgacggtat cgat
//
