pLY-4 vector (V015923)

LOCUS       V015923                 6968 bp    DNA     circular SYN 11-APR-2020
DEFINITION  Exported.
ACCESSION   V015923
VERSION     V015923
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Li Y, Ai Y, Zhang J, Fei J, Liu B, Wang J, Li M, Zhao Q, Song J.
  TITLE     A novel expression vector for Corynebacterium glutamicum with an
            auxotrophy complementation system
  JOURNAL   Plasmid 107, 102476 (2020)
   PUBMED   31758959
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Li Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2019) School of Food Science and Engineering,
            Dalian Ocean University, 52 Heishijiao Street, Dalian, Liaoning
            116023, China
REFERENCE   3  (bases 1 to 6968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
            107, 102476 (2020)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-OCT-2019) School of Food Science and Engineering, Dalian Ocean
            University, 52 Heishijiao Street, Dalian, Liaoning 116023, China"
FEATURES             Location/Qualifiers
     source          1..6968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      299..385
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      477..504
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        523..614
                     /label="AmpR promoter"
     rep_origin      complement(1001..1589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      1753..4308
                     /label="pBL1"
                     /note="pBL1"
     CDS             complement(4443..5099)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     regulatory      5165..5259
                     /label="psiGA1"
                     /note="psiGA1"
                     /regulatory_class="promoter"
     CDS             5677..6759
                     /gene="alr"
                     /label="Alanine racemase"
                     /note="Alanine racemase from Corynebacterium glutamicum
                     (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 /
                     LMG 3730 / NCIMB 10025). Accession#: Q8RSU9"
     protein_bind    6944..6960
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."