pLY-4 vector (V015923)

MSDS

Basic Vector Information

Vector Name:: pLY-4

Antibiotic Resistance:: Chloramphenicol

Length:: 6968 bp

Type:: Shuttle vector

Replication origin:: ori

Source/Author:: Li Y, Ai Y, Zhang J, Fei J

pLY-4 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLY-4 vector Sequence

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LOCUS       62056_16380        6968 bp DNA     circular SYN 11-APR-2020
DEFINITION  Shuttle vector pLY-4, complete sequence.
ACCESSION   MN562271
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Li Y, Ai Y, Zhang J, Fei J, Liu B, Wang J, Li M, Zhao Q, Song J.
  TITLE     A novel expression vector for Corynebacterium glutamicum with an 
            auxotrophy complementation system
  JOURNAL   Plasmid 107, 102476 (2020)
  PUBMED    31758959
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Li Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2019) School of Food Science and Engineering, 
            Dalian Ocean University, 52 Heishijiao Street, Dalian, Liaoning 
            116023, China
REFERENCE   3  (bases 1 to 6968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            107, 102476 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-OCT-2019) School of Food Science and Engineering, Dalian Ocean 
            University, 52 Heishijiao Street, Dalian, Liaoning 116023, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      299..385
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      477..504
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        523..614
                     /label=AmpR promoter
     rep_origin      complement(1001..1589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1753..4308
                     /label=pBL1
                     /note="pBL1"
     CDS             complement(4443..5099)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      5165..5259
                     /label=psiGA1
                     /note="psiGA1"
                     /regulatory_class="promoter"
     CDS             5677..6759
                     /gene="alr"
                     /label=alr
                     /note="Alanine racemase from Corynebacterium glutamicum
                     (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / 
                     LMG 3730 / NCIMB 10025). Accession#: Q8RSU9"
     protein_bind    6944..6960
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

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