LOCUS       V015923                 6968 bp    DNA     circular SYN 11-APR-2020
DEFINITION  Exported.
ACCESSION   V015923
VERSION     V015923
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Li Y, Ai Y, Zhang J, Fei J, Liu B, Wang J, Li M, Zhao Q, Song J.
  TITLE     A novel expression vector for Corynebacterium glutamicum with an
            auxotrophy complementation system
  JOURNAL   Plasmid 107, 102476 (2020)
   PUBMED   31758959
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Li Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2019) School of Food Science and Engineering,
            Dalian Ocean University, 52 Heishijiao Street, Dalian, Liaoning
            116023, China
REFERENCE   3  (bases 1 to 6968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
            107, 102476 (2020)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-OCT-2019) School of Food Science and Engineering, Dalian Ocean
            University, 52 Heishijiao Street, Dalian, Liaoning 116023, China"
FEATURES             Location/Qualifiers
     source          1..6968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      299..385
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      477..504
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        523..614
                     /label="AmpR promoter"
     rep_origin      complement(1001..1589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      1753..4308
                     /label="pBL1"
                     /note="pBL1"
     CDS             complement(4443..5099)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     regulatory      5165..5259
                     /label="psiGA1"
                     /note="psiGA1"
                     /regulatory_class="promoter"
     CDS             5677..6759
                     /gene="alr"
                     /label="Alanine racemase"
                     /note="Alanine racemase from Corynebacterium glutamicum
                     (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 /
                     LMG 3730 / NCIMB 10025). Accession#: Q8RSU9"
     protein_bind    6944..6960
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattaagctt atgcggccgc atctcgagat agatctatgc tagcatctgc aggtcgactc
       61 tagaggatcc ccgggtaccg agctcgaatt cagcttggct gttttggcgg atgagagaag
      121 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
      181 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      241 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      361 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      421 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
      481 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa
      541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttgcttcc tcgctcactg
      841 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      901 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      961 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1021 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1081 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1141 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     1201 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1261 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1321 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1381 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1441 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1501 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1561 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1621 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1681 tcttcaccta gatccttttg gggtgggcga agaactccag catgagatcc ccgcgctgga
     1741 ggatcatcca gccattcggg gtcgttcact ggttcccctt tctgatttct ggcatagaag
     1801 aacccccgtg aactgtgtgg ttccgggggt tgctgatttt tgcgagactt ctcgcgcaat
     1861 tccctagctt aggtgaaaac accatgaaac actagggaaa cacccatgaa acacccatta
     1921 gggcagtagg gcggcttctt cgtctagggc ttgcatttgg gcggtgatct ggtctttagc
     1981 gtgtgaaagt gtgtcgtagg tggcgtgctc aatgcactcg aacgtcacgt catttaccgg
     2041 gtcacggtgg gcaaagagaa ctagtgggtt agacattgtt ttcctcgttg tcggtggtgg
     2101 tgagcttttc tagccgctcg gtaaacgcgg cgatcatgaa ctcttggagg ttttcaccgt
     2161 tctgcatgcc tgcgcgcttc atgtcctcac gtagtgccaa aggaacgcgt gcggtgacca
     2221 cgacgggctt agcctttgcc tgcgcttcta gtgcttcgat ggtggcttgt gcctgcgctt
     2281 gctgcgcctg tagtgcctgt tgagcttctt gtagttgctg ttctagctgt gccttggttg
     2341 ccatgcttta agactctagt agctttcctg cgatatgtca tgcgcatgcg tagcaaacat
     2401 tgtcctgcaa ctcattcatt atgtgcagtg ctcctgttac tagtcgtaca tactcatatt
     2461 tacctagtct gcatgcagtg catgcacatg cagtcatgtc gtgctaatgt gtaaaacatg
     2521 tacatgcaga ttgctggggg tgcagggggc ggagccaccc tgtccatgcg gggtgtgggg
     2581 cttgccccgc cggtacagac agtgagcacc ggggcaccta gtcgcggata ccccccctag
     2641 gtatcggaca cgtaaccctc ccatgtcgat gcaaatcttt aacattgagt acgggtaagc
     2701 tggcacgcat agccaagcta ggcggccacc aaacaccact aaaaattaat agtccctaga
     2761 caagacaaac ccccgtgcga gctaccaact catatgcacg ggggccacat aacccgaagg
     2821 ggtttcaatt gacaaccata gcactagcta agacaacggg cacaacaccc gcacaaactc
     2881 gcactgcgca accccgcaca acatcgggtc taggtaacac tgagtaacac tgaaatagaa
     2941 gtgaacacct ctaaggaacc gcaggtcaat gagggttcta aggtcactcg cgctagggcg
     3001 tggcgtaggc aaaacgtcat gtacaagatc accaatagta aggctctggc ggggtgccat
     3061 aggtggcgca gggacgaagc tgttgcggtg tcctggtcgt ctaacggtgc ttcgcagttt
     3121 gagggtctgc aaaactctca ctctcgctgg gggtcacctc tggctgaatt ggaagtcatg
     3181 ggcgaacgcc gcattgagct ggctattgct actaagaatc acttggcggc gggtggcgcg
     3241 ctcatgatgt ttgtgggcac tgttcgacac cgatatgtca tgcgcatgcg tagcaaacat
     3301 tgtcctgcaa ctcattcatt atgtgcagtg gtgaaaacat ctcagtggaa gaaagaacgt
     3361 gcacggtacg gggtggagca cacctatagt gactatgagg tcacagactc ttgggcgaac
     3421 ggttggcact tgcaccgcaa catgctgttg ttcttggatc gtccactgtc tgacgatgaa
     3481 ctcaaggcgt ttgaggattc catgttttcc cgctggtctg ctggtgtggt taaggccggt
     3541 atggacgcgc cactgcgtga gcacggggtc aaacttgatc aggtgtctac ctggggtgga
     3601 gacgctgcga aaatggcaac ctacctcgct aagggcatgt ctcaggaact gactggctcc
     3661 gctactaaaa ccgcgtctaa ggggtcgtac acgccgtttc agatgttgga tatgttggcc
     3721 gatcaaagcg acgccggcga ggatatggac gctgttttgg tggctcggtg gcgtgagtat
     3781 gaggttggtt ctaaaaacct gcgttcgtcc tggtcacgtg gggctaagcg tgctttgggc
     3841 attgattaca tagacgctga tgtacgtcgt gaaatggaag aagaactgta caagctcgcc
     3901 ggtctggaag caccggaacg ggtcgaatca acccgcgttg ctgttgcttt ggtgaagccc
     3961 gatgattgga aactgattca gtctgatttc gcggttaggc agtacgttct cgattgcgtg
     4021 gataaggcta aggacgtggc cgctgcgcaa cgtgtcgcta atgaggtgct ggcaagtctg
     4081 ggtgtggatt ccaccccgtg catgatcgtt atggatgatg tggacttgga cgcggttctg
     4141 cctactcatg gggacgctac taagcgtgat ctgaatgcgg cggtgttcgc gggtaatgag
     4201 cagactattc ttcgcaccca ctaaaagcgg cataaacccc gttcgatatt ttgtgcgatg
     4261 aatttatggt caatgtcgcg ggggcaaact atgatgggtc ttgttgttgg cgtcccggaa
     4321 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     4381 caggttgggc gtcgcttggt cggtcatttc gaagggcacc aataactgcc ttaaaaaaat
     4441 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     4501 tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     4561 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     4621 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     4681 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     4741 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     4801 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     4861 ccgtctttca ttgccatacg gaactccgga tgagcattca tcaggcgggc aagaatgtga
     4921 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     4981 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     5041 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     5101 ttagcttcct tagctcctga aaatctcgtc gaagctcggc ggatttgtcc tactcaagct
     5161 gatccgacaa aatccacaca ttatcccagg tgtccggatc ggtcaaatac gctgccagct
     5221 catagaccgt atccaaagca tccggggctg atccccggcg ccagggtggt ttttcttttc
     5281 accagtgaga cgggcaacag ctgattgccc tcgcgcgcgc ctttgtggtc tggcatgaag
     5341 ccattttcgg tgtctggggc ggcaacagcg atgccccagg acgcgaggtg tcgcaaagtt
     5401 tggtggtagt acttgatgga tttcatccag tcgtggccga aagctacacc tgggatgccg
     5461 tcgccttctg ctggggtgta gattttgccc gggatgccgg cgtagttcat atcgcctacc
     5521 agcacgcggt gcggtccgcg cttggacagt ttggacaggt gtttgttcag attctcagcc
     5581 acgtgtttaa ggatagttga aagcgtgggg caatactggc actaaccccg gcaccaatcg
     5641 tatttctgtc cgcggttggt ggcacaatag ttcaacatga acttgctgac caccaaaatt
     5701 gacctggatg ccatcgccca taacacgagg gtgcttaaac aaatggcggg tccggcgaag
     5761 ctgatggcgg tggtgaaggc gaatgcatat aaccatggcg tagagaaggt cgctccggtt
     5821 attgctgctc atggtgcgga tgcgtttggt gtggcaactc ttgcggaggc tatgcagttg
     5881 cgtgatatcg gcatcagcca agaggttttg tgttggattt ggacaccgga gcaggatttc
     5941 cgcgccgcca ttgatcgcaa tattgatttg gctgttattt ctcccgcgca tgccaaagcc
     6001 ttgatcgaaa ctgatgcgga gcatattcgg gtgtccatca agattgattc tgggttgcat
     6061 cgttcgggtg tggatgagca ggagtgggag ggcgtgttca gcgcgttggc tgctgccccg
     6121 cacattgagg tcacgggcat gttcacgcac ttggcgtgcg cggatgagcc agagaatccg
     6181 gaaactgatc gccaaattat tgcttttcga cgcgcccttg cgctcgcccg caagcacggg
     6241 cttgagtgcc cggtcaacca cgtatgcaac tcacctgcat tcttgactcg atctgattta
     6301 cacatggaga tggtccgacc gggtttggcc ttttatgggt tggaacccgt ggcgggactg
     6361 gagcatggtt tgaagccggc gatgacgtgg gaggcgaagg tgagcgtcgt aaagcaaatt
     6421 gaagctggac aaggcacttc ctatggcctg acctggcgcg ctgaggatcg cggctttgtg
     6481 gctgtggtgc ctgcgggcta tgccgatggc atgccgcggc atgcccaggg gaaattctcc
     6541 gtcacgattg atggcctgga ctatccgcag gttgggcgcg tatgcatgga tcagttcgtt
     6601 atttctttgg gcgacaatcc acacggcgtg gaagctgggg cgaaggccgt gatattcggt
     6661 gagaatgggc atgacgcaac tgattttgcg gagcgtttag acaccattaa ctatgaggta
     6721 gtgtgccgac caaccggccg aactgtccgc gcatatgttt aagtgaatac gtttaaggag
     6781 cagcaatgaa atctgagttt ccggtatccg gcacgaggcg ttttgagcat gccgcagata
     6841 cccaaaattt tggggaagaa ttaggcaggc atctagaagc tggcgatgtg gtgattttgt
     6901 tgagctgttg acaattaatc atcgtgtggt accatgtgtg gaattgtgag cggataacaa
     6961 ttgcaatt
//
