Basic Vector Information
- Vector Name:
- AAV-CaMKII-GCaMP6f
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6767 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR
- Promoter:
- CaMKII
AAV-CaMKII-GCaMP6f vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AAV-CaMKII-GCaMP6f vector Sequence
LOCUS 62056_211 6767 bp DNA circular SYN 17-NOV-2020
DEFINITION Cloning vector AAV-CaMKII-GCaMP6f, complete sequence.
ACCESSION MW139900
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6767)
AUTHORS Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T,
White JA, Berg J, Han X.
TITLE CamKIIalpha Positive Interneurons Identified via A microRNA Based
Viral Gene Targeting Strategy
JOURNAL J Neurosci (2020) In press
PUBMED 33158963
REFERENCE 2 (bases 1 to 6767)
AUTHORS Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T,
White JA, Berg J, Han X.
TITLE Direct Submission
JOURNAL Submitted (16-OCT-2020) Biomedical Engineering, Boston University,
44 Cummington Mall, Boston, MA 02215, USA
REFERENCE 3 (bases 1 to 6767)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci
(2020) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-OCT-2020) Biomedical Engineering, Boston University, 44
Cummington Mall, Boston, MA 02215, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6767
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 228..249
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 264..294
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 302..318
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 326..342
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
repeat_region 380..480
promoter 576..1864
/label=CaMKII promoter
/note="promoter for the mouse calcium/calmodulin-dependent
kinase II alpha subunit"
CDS 1884..3233
/label=GCaMP6f
/note="improved fluorescent protein-based calcium sensor
(Chen et al., 2013)"
misc_feature 3276..3864
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3867..3883)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal complement(3913..4034)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
repeat_region 4137..4326
primer_bind complement(4344..4360)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4502..4957
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4983..5087
/label=AmpR promoter
CDS 5088..5945
/label=AmpR
/note="beta-lactamase"
rep_origin 6119..6707
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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