LOCUS       62056_211        6767 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector AAV-CaMKII-GCaMP6f, complete sequence.
ACCESSION   MW139900
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T, 
            White JA, Berg J, Han X.
  TITLE     CamKIIalpha Positive Interneurons Identified via A microRNA Based 
            Viral Gene Targeting Strategy
  JOURNAL   J Neurosci (2020) In press
  PUBMED    33158963
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T, 
            White JA, Berg J, Han X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) Biomedical Engineering, Boston University, 
            44 Cummington Mall, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 6767)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-OCT-2020) Biomedical Engineering, Boston University, 44 
            Cummington Mall, Boston, MA 02215, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    228..249
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        264..294
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   380..480
     promoter        576..1864
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             1884..3233
                     /label=GCaMP6f
                     /note="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
     misc_feature    3276..3864
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(3867..3883)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(3913..4034)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4137..4326
     primer_bind     complement(4344..4360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4502..4957
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4983..5087
                     /label=AmpR promoter
     CDS             5088..5945
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6119..6707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaagatctg aattccatta tggccttagg tcacttcatc
      601 tccatggggt tcttcttctg attttctaga aaatgagatg ggggtgcaga gagcttcctc
      661 agtgacctgc ccagggtcac atcagaaatg tcagagctag aacttgaact cagattacta
      721 atcttaaatt ccatgccttg ggggcatgca agtacgatat acagaaggag tgaactcatt
      781 agggcagatg accaatgagt ttaggaaaga agagtccagg gcagggtaca tctacaccac
      841 ccgcccagcc ctgggtgagt ccagccacgt tcacctcatt atagttgcct ctctccagtc
      901 ctaccttgac gggaagcaca agcagaaact gggacaggag ccccaggaga ccaaatcttc
      961 atggtccctc tgggaggatg ggtggggaga gctgtggcag aggcctcagg aggggccctg
     1021 ctgctcagtg gtgacagata ggggtgagaa agcagacaga gtcattccgt cagcattctg
     1081 ggtctgtttg gtacttcttc tcacgctaag gtggcggtgt gatatgcaca atggctaaaa
     1141 agcagggaga gctggaaaga aacaaggaca gagacagagg ccaagtcaac cagaccaatt
     1201 cccagaggaa gcaaagaaac cattacagag actacaaggg ggaagggaag gagagatgaa
     1261 ttagcttccc ctgtaaacct tagaacccag ctgttgccag ggcaacgggg caatacctgt
     1321 ctcttcagag gagatgaagt tgccagggta actacatcct gtctttctca aggaccatcc
     1381 cagaatgtgg cacccactag ccgttaccat agcaactgcc tctttgcccc acttaatccc
     1441 atcccgtctg ttaaaagggc cctatagttg gaggtggggg aggtaggaag agcgatgatc
     1501 acttgtggac taagtttgtt cgcatcccct tctccaaccc cctcagtaca tcaccctggg
     1561 ggaacagggt ccacttgctc ctgggcccac acagtcctgc agtattgtgt atataaggcc
     1621 agggcaaaga ggagcaggtt ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc
     1681 ggggcaacgg gaacagggcg tttcggaggt ggttgccatg gggacctgga tgctgacgaa
     1741 ggctcgcgag gctgtgagca gccacagtgc cctgctcaga agccccaagc tcgtcagtca
     1801 agccggttct ccgtttgcac tcaggagcac gggcaggcga gtggccccta gttctggggg
     1861 cagcggggcg cgccgccgcc accatgggtt ctcatcatca tcatcatcat ggtatggcta
     1921 gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat aaggatctcg
     1981 ccaccatggt cgactcatca cgtcgtaagt ggaataagac aggtcacgca gtcagagcta
     2041 taggtcggct gagctcactc gagaacgtct atatcaaggc cgacaagcag aagaacggca
     2101 tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc
     2161 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2221 tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2281 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta
     2341 ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2401 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg
     2461 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2521 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     2581 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc
     2641 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2701 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2761 tcctggggca caagctggag tacaacctgc cggaccaact gactgaagag cagatcgcag
     2821 aatttaaaga ggaattctcc ctatttgaca aggacgggga tgggacaata acaaccaagg
     2881 agctggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag ctgcaggaca
     2941 tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag ttcctgacaa
     3001 tgatggcaag aaaaatgaaa tacagggaca cggaagaaga aattagagaa gcgttcggtg
     3061 tgtttgataa ggatggcaat ggctacatca gtgcagcaga gcttcgccac gtgatgacaa
     3121 accttggaga gaagttaaca gatgaagagg ttgatgaaat gatcagggaa gcagacatcg
     3181 atggggatgg tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg aagtgaacta
     3241 gtgcggccgc cctgcaggca tgcaagctta tcgataatca acctctggat tacaaaattt
     3301 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3361 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3421 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3481 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3541 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3601 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3661 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3721 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3781 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3841 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgacccggg cggccgcttc
     3901 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3961 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     4021 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     4081 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     4141 ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa
     4201 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4261 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4321 cgcagcctta attaacctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4381 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4441 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4501 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4561 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4621 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4681 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4741 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4801 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4861 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4921 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4981 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5041 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5101 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5161 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5221 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5281 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5341 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5401 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5461 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5521 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5581 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5641 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5701 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5761 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5821 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6241 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatg
//
