pSJ+SP+AaeUPO1 vector (V015904)

LOCUS       V015904                 8205 bp    DNA     circular SYN 28-AUG-2021
DEFINITION  Exported.
ACCESSION   V015904
VERSION     V015904
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Jacob S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2021) Institute for Biotechnology and Drug
            Research, IBWF, Hanns-Dieter-Husch-Weg 17, Mainz, RLP 55128, Germany
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (26-JUL-2021) Institute for Biotechnology and Drug Research, IBWF,
            Hanns-Dieter-Husch-Weg 17, Mainz, RLP 55128, Germany"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1473..2099)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    2251..2275
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3573..4682
                     /gene="APO1"
                     /label="Aromatic peroxygenase"
                     /note="Aromatic peroxygenase from Cyclocybe aegerita.
                     Accession#: B9W4V6"
     terminator      4710..4962
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4971..5324
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             5347..5895
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     misc_feature    5982..6006
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6169..6960)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6987..7575
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      complement(7913..8107)
                     /direction=LEFT
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(8176..8205,1..1041))
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"