LOCUS       V015904                 8205 bp    DNA     circular SYN 28-AUG-2021
DEFINITION  Exported.
ACCESSION   V015904
VERSION     V015904
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Jacob S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2021) Institute for Biotechnology and Drug
            Research, IBWF, Hanns-Dieter-Husch-Weg 17, Mainz, RLP 55128, Germany
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (26-JUL-2021) Institute for Biotechnology and Drug Research, IBWF,
            Hanns-Dieter-Husch-Weg 17, Mainz, RLP 55128, Germany"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1473..2099)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    2251..2275
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3573..4682
                     /gene="APO1"
                     /label="Aromatic peroxygenase"
                     /note="Aromatic peroxygenase from Cyclocybe aegerita.
                     Accession#: B9W4V6"
     terminator      4710..4962
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4971..5324
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             5347..5895
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     misc_feature    5982..6006
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6169..6960)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6987..7575
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      complement(7913..8107)
                     /direction=LEFT
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(8176..8205,1..1041))
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
       61 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
      121 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
      181 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccggggtcga tcaaaaagta
      241 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
      301 atcagcaagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
      361 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
      421 ggtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
      481 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
      541 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
      601 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggcggggcc
      661 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
      721 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
      781 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
      841 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
      901 cccttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
      961 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     1021 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     1081 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     1141 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     1201 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     1261 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     1321 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     1381 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     1441 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     1501 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     1561 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     1621 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     1681 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     1741 tccaccaggt cggcggtggc ccaaatgtcg taagggcttg gctgcaccgg aatcagcacg
     1801 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     1861 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
     1921 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
     1981 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
     2041 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
     2101 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
     2161 gcaagctgtt ttactcaaat acacatcacc tttttagatg atcagtgatt ttgtgccgag
     2221 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
     2281 gacgcttaga caacttaata acacattgcg gacgtcttta atgtactgaa tttagttact
     2341 gatcactgat taagtactga tatcggtacg tcgactgaga gcgagaaaaa aaaactcttc
     2401 gtcggattat catgccgcaa gctttatgca gttgtatttg tgtagttttt gagtttgatg
     2461 ttcacaagta cagtacgtgg taagtctttc tttcccatga gatactactc tcccttgtta
     2521 cagacttgct gacaccctgt tctccttttt cttttctaat atttgaacac atgagccaaa
     2581 atacatacat acatgaaaaa gcacaatgat acgaccgtgt ggcacagcac ctcagataag
     2641 caacgggtgg cgatgcaatg acatacatcg aattagagta ccgctaatgt tcgtgagggg
     2701 caaatgcaaa cggccaaacc aaagagtaac agggatctaa acctgtaccg tgagtcactt
     2761 ttcccccgcg acaagggggg cggtcttaac aaccgcggga cgaccagatc ctccaaatct
     2821 tgcgacgtca atgtctgcag gaggtgtccg cttggtgccc aatgctactc tacagtcttg
     2881 gcttatacga cacgtagtgg ccccgcgctg gccggagctt ttttccatag taactagatc
     2941 atggtaacgc gttgtggtga ggtgagaaga ggacaagctg ttgtcgaaaa gattgtagta
     3001 aaaaagacaa tgaccgtgta gctgtttgtg aggaggagct tccaaactca atcatcctct
     3061 agactagaac tagaatctac agtagtacct agctatgaag ttacttgaag ggaggagctt
     3121 gaaagtgaac aaccaacctc cagggccgga cctgaatcta ggctcatctc aaggtgagat
     3181 ggaacacttc catgcccggt tcctatcggc caatgcgagg aggaggggca gacactttcg
     3241 ttgccacaga aaaaaaaatc ttttttcgtt tgttttgcgc aaggcgggca aggtggggca
     3301 gcctgtggcc aacgtgaatg gaggggtaaa aaaaaagagg gagccatctg cggggcaccg
     3361 tcgatgtata tcttttcatc ctccgcgcaa aactttgcac tacgaaccct ttctcttccc
     3421 tttctcgatc ctcgtctttg catacctgaa atcatccgca tttcaacctg caatctttaa
     3481 acacacacat tatcatcaaa aagagagcac caaaccgcca catgaagttc ctcaccccgc
     3541 tcgccgccct tctcagcacc gcctcggcgg ccaaatattt tcccctgttc ccaaccttgg
     3601 tcttcgcagc gagggtcgtt gcttttcctg cctacgcctc attggccggc ctcagccagc
     3661 aggaattgga cgctataatc ccaacactcg aggcccgaga gccaggatta cctcctggtc
     3721 ctctcgagaa tagctctgca aagttggtga acgacgaggc tcacccatgg aagccgcttc
     3781 gacctggcga tattcgtgga ccttgccctg gtctcaatac tctggcatct cacgggtacc
     3841 tcccgagaaa tggcgttgca accccggtgc aaataataaa cgcggttcag gaaggactca
     3901 atttcgacaa tcaagccgca gtcttcgcca catatgcggc ccaccttgtg gacggcaatc
     3961 tcattacgga cttgctgagc atcggacgca agacgcggct cactgggcct gatccaccac
     4021 cccccgcttc cgttggtgga ctcaatgagc atggcacctt cgaaggcgac gccagtatga
     4081 cccgaggtga cgcattcttt ggcaacaacc acgatttcaa tgagacgctc ttcgaacagt
     4141 tggttgacta cagcaaccga tttggaggag gaaaatacaa tcttaccgtc gcgggggagc
     4201 tccgtttcaa gcgcattcaa gactccattg cgaccaaccc caatttctcc tttgttgact
     4261 ttaggttctt tactgcttac ggcgagacca ccttccccgc gaatcttttt gtggatgggc
     4321 gcagggacga cggccagcta gatatggatg ctgcacggag ttttttccaa ttcagccgta
     4381 tgcctgacga tttcttccgc gcacccagcc cgagaagtgg cacaggagtc gaggtagtta
     4441 tacaggctca tcctatgcag cccggaagaa atgtcggcaa gatcaacagc tacaccgtcg
     4501 acccaacatc ctctgacttt tccaccccct gcttgatgta cgagaaattc gtcaacataa
     4561 cggtcaagtc actctacccg aatccgacgg tgcagcttcg caaagccctt aatacgaatc
     4621 tcgatttctt cttccaggga gtcgccgctg gatgtaccca ggtcttccca tacgggcgag
     4681 attgaaagta aagcggccgc ccggctgcag atcgttcaaa catttggcaa taaagtttct
     4741 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     4801 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4861 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     4921 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgtcgacgt ttgatattga
     4981 aggagcactt tttgggcttg gctggagcta gtggaggtca acaatgaatg cctattttgg
     5041 tttagtcgtc caggcggtga gcacaaaatt tgtgtcgttt gacaagatgg ttcatttagg
     5101 caactggtca gatcagcccc acttgtagca gtagcggcgg cgctcgaagt gtgactctta
     5161 ttagcagaca ggaacgagga cattattatc atctgctgct tggtgcacga taacttggtg
     5221 cgtttgtcaa gcaaggtaag tgaacgaccc ggtcatacct tcttaagttc gcccttcctc
     5281 cctttatttc agattcaatc tgacttacct attctaccca agcaacgctt cgattaggaa
     5341 gtaaccatga gcccagaacg acgcccggcc gacatccgcc gtgccaccga ggcggacatg
     5401 ccggcggtct gcaccatcgt caaccactac atcgagacaa gcacggtcaa cttccgtacc
     5461 gagccgcagg aaccgcagga gtggacggac gacctcgtcc gtctgcggga gcgctatccc
     5521 tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg tccctggaag
     5581 gcacgcaacg cctacgactg gacggccgaa tcgaccgtgt acgtctcccc ccgccaccag
     5641 cggacgggac tgggctccac gctctacacc cacctgctga agtccctgga ggcacagggc
     5701 ttcaagagcg tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg catgcacgag
     5761 gcgctcggat atgccccccg cggcatgctg cgggcggccg gcttcaagca cgggaactgg
     5821 catgacgtgg gtttctggca gctggacttc agcctgccgg ttccgccccg tccggtcctg
     5881 cccgtcaccg agatttagaa cgtcgacccg gccgtttaaa cttagttact aatcagtgat
     5941 cagattgtcg tttcccgcct tcactttaaa ctatcagtgt ttgacaggat atattggcgg
     6001 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     6061 ggtttatccg ttcgtccatt tgtatgtcaa tattgggggg gggggaaagc cacgttgtgt
     6121 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatctaaa acaattcatc
     6181 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
     6241 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
     6301 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
     6361 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
     6421 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
     6481 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
     6541 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
     6601 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
     6661 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
     6721 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
     6781 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
     6841 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
     6901 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
     6961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7681 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7741 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     7801 tcacaccgca taggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     7861 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     7921 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     7981 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     8041 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     8101 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     8161 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcag
//
