Basic Vector Information
- Vector Name:
- pNRVL-LEU2-GFP
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7445 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Nemeth T.
pNRVL-LEU2-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNRVL-LEU2-GFP vector Sequence
LOCUS 62056_18235 7445 bp DNA circular SYN 03-MAR-2020
DEFINITION Expression vector pNRVL-LEU2-GFP, complete sequence.
ACCESSION MT001911
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7445)
AUTHORS Nemeth T.
TITLE Direct Submission
JOURNAL Submitted (28-JAN-2020) Department of Microbiology, University of
Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE 2 (bases 1 to 7445)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(28-JAN-2020) Department of Microbiology, University of Szeged,
Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT ##Assembly-Data-START##
Assembly Method :: CLC Genomics Workbench v. v11
Sequencing Technology :: IonTorrent
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7445
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(268..295)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(387..473)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 570..575
/label=StuI site for cassette release
/note="StuI site for cassette release"
misc_feature 576..1039
/note="site of recombination; CpNEUT5LUpup from C.
parapsilosis"
regulatory 1096..1943
/label=Candida maltosa LEU2 promoter
/note="Candida maltosa LEU2 promoter"
/regulatory_class="promoter"
CDS 1944..3062
/gene="LEU2"
/label=LEU2
/note="3-isopropylmalate dehydrogenase from Candida
maltosa. Accession#: P07139"
regulatory 3066..3280
/label=Candida maltosa LEU2 terminator
/note="Candida maltosa LEU2 terminator"
/regulatory_class="terminator"
regulatory complement(3333..3573)
/label=Saccharomyces cerevisiae URA3 terminator
/note="Saccharomyces cerevisiae URA3 terminator"
/regulatory_class="terminator"
protein_bind 3594..3618
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(3622..4335)
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
protein_bind complement(4336..4360)
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
regulatory complement(4367..5216)
/label=Candida albicans TDH3 promoter
/note="Candida albicans TDH3 promoter"
/regulatory_class="promoter"
misc_feature 5223..5673
/note="site of recombination; CpNEUT5LDowndown from C.
parapsilosis"
misc_feature 5674..5679
/label=StuI site for cassette release
/note="StuI site for cassette release"
promoter complement(5687..5705)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5710..5726)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5839..6645
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 6795..7383
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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