pNRVL-LEU2-GFP vector (V015897)

LOCUS       V015897                 7445 bp    DNA     circular SYN 03-MAR-2020
DEFINITION  Exported.
ACCESSION   V015897
VERSION     V015897
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-JAN-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..7445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    570..575
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1096..1943
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     CDS             1944..3062
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      3066..3280
                     /label="Candida maltosa LEU2 terminator"
                     /note="Candida maltosa LEU2 terminator"
                     /regulatory_class="terminator"
     regulatory      complement(3333..3573)
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     protein_bind    3594..3618
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3622..4335)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     protein_bind    complement(4336..4360)
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(4367..5216)
                     /label="Candida albicans TDH3 promoter"
                     /note="Candida albicans TDH3 promoter"
                     /regulatory_class="promoter"
     misc_feature    5223..5673
                     /note="site of recombination; CpNEUT5LDowndown from C.
                     parapsilosis"
     misc_feature    5674..5679
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     promoter        complement(5687..5705)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5710..5726)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5839..6645
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6795..7383
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"