LOCUS       V015897                 7445 bp    DNA     circular SYN 03-MAR-2020
DEFINITION  Exported.
ACCESSION   V015897
VERSION     V015897
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-JAN-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..7445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    570..575
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1096..1943
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     CDS             1944..3062
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      3066..3280
                     /label="Candida maltosa LEU2 terminator"
                     /note="Candida maltosa LEU2 terminator"
                     /regulatory_class="terminator"
     regulatory      complement(3333..3573)
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     protein_bind    3594..3618
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3622..4335)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     protein_bind    complement(4336..4360)
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(4367..5216)
                     /label="Candida albicans TDH3 promoter"
                     /note="Candida albicans TDH3 promoter"
                     /regulatory_class="promoter"
     misc_feature    5223..5673
                     /note="site of recombination; CpNEUT5LDowndown from C.
                     parapsilosis"
     misc_feature    5674..5679
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     promoter        complement(5687..5705)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5710..5726)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5839..6645
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6795..7383
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagtcaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacactagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgcagc tcggatccac tagtaacggc cgccagtgtg
     1081 ctggaattcg cccttggatc caatcatcac tggtgggggt caagaaagag gtttgagatc
     1141 aggtactttg gcaccaccat tagtagctgg gtttggtgaa gctgcaagat taatgaaaca
     1201 agaatctgca tttgacaaga aacatattga gaggttatct actaaattga agaacggttt
     1261 gttatccatt ccatctactc agtttaacgg ttgtaataac ccaacatatc aatacccagg
     1321 ttgtgtgaat gtttcctttg catacattga aggggaatct ttgttaatgg cattgaaaga
     1381 tattgcattg agttctggtt ctgcatgtac ctctgcatct ttggaaccat cgtatgtttt
     1441 acatgctttg ggtgccgatg atgccttggc tcattcttcc attagattcg gtattggtag
     1501 attcaccact gaagcagaag ttgactatgt tattcaagca attaatgaaa gagttgattt
     1561 cttgagaaag atgtctccat tatgggaaat ggttcaagaa ggtattgact tgaactctat
     1621 cgaatggagt ggacattaag gttttttgtt tttttgtaat ttgtgttgtt atgtgtaatt
     1681 atcgtgtata gagtaatttc ggaaattgta ctttcgttca tgtatctgaa ccagatgaat
     1741 aatacgttcg tattgttgaa gaatgactaa ctcctgtaac ggcgtgcgtg ggttccgcag
     1801 ccgggttcaa tttttttttt cttgttcact ataaaaatat ataataaacg aaaaaaatcc
     1861 gagggactca ggctatgaaa tttttcactc aagaattttt tttccttcat ccttaattcc
     1921 tttattcact acccaaaccc acaatgtcag ttaaaactaa aactattaca attttaccag
     1981 gtgatcacgt cggtaccgaa attgtcaatg aagctatcaa agttttagaa gcaattgaag
     2041 ctgccactcc ttaccaaaaa atccatttcg atttcaaaca tcatttaatc ggtggtgctg
     2101 ccattgatgc cactggtgtc ccattgccag atgacgcact tgaaagtgcc aaaaaatctg
     2161 atgccgtctt attgggtgct gttggtggac caaaatgggg gactggcgcc gttcgtccag
     2221 aacaaggttt attaaagatt cgtaaagaat tgaaccttta tgctaacatc agaccatgta
     2281 actttgccag tgactcctta ttggaattat ccccattgag accagaagtc gttaaaggta
     2341 caaacttgat cattgttcgt gaattagttg gtggtattta tttcggtgat cgtgaagaac
     2401 aagaagaaag tgaagataaa caaaccgcat gggatactga aaaatacacc gtcgatgaag
     2461 tcaccagaat cacccgtatg gctgccttca tggctttaca acataaccca ccattaccaa
     2521 tctggtcatt ggataaggct aatgttttag cttcttctag attatggaga agaactgtcg
     2581 ataaagtgat ttctgaagag ttcccaactt tgtctgttca acatcaattg attgactccg
     2641 ccgccatgat tttaattcaa aacccaacca aattgaatgg tataatcatc acttctaaca
     2701 tgtttggtga tatcatttcc gatgaagctt cagttattcc aggttccttg ggtttattac
     2761 catctgcatc tttggcttca ttgccagata ccaacaccgc ttttggtctt tatgaaccat
     2821 gtcacggttc tgctcctgat ttaccagcca ataaagttaa cccaattgct actattttat
     2881 ctgctgcttc tatgttgaga ttatctttgg attgtgtgaa agaagctgaa gctttagaag
     2941 aagccgtgaa acaagtcttg gataaaggta tcagaactgc agatttaaga ggtaccagtt
     3001 cagctactga agttggtgac gcgattgtcg aagctgttac taaaatctta aaagaaaaag
     3061 cttaagtaaa gtatacacaa aatttaacga atattattaa tacaaaatga acaccaccta
     3121 aatacttttt tttaaaaatg taccgactct tctaaaaatt gcgataattc aatatcggaa
     3181 atttctctca ccggtttact tggatcttcg gaaataaatc tatttgaagt aatggtagcc
     3241 tcacttgatt ctgattggct ctttctagac atgggtaggt aagggcgaat tctgcagata
     3301 tccatcacac tggcgcgtga attcgatctg ctatgcgtcc atctttacag tcctgtctta
     3361 ttgttcttga tttgtgcccc gtaaaatact gttacttggt tctggcgagg tattggatag
     3421 ttccttttta taaaggccat gaagcttttt ctttccaatt tttttttttc gtcattatag
     3481 aaatcattac gaccgagatt cccgggtaat aactgatata attaaattga agctctaatt
     3541 tgtgagttta gtatacatgc atttacttat aatgctattt attcatgcta gcaaccactt
     3601 tgtacaagaa agctgggttt atttgtacaa ttcatccata ccatgggtaa taccagcagc
     3661 agtaacaaat tctaacaaga ccatgtggtc tctcttttcg tttggatctt tggataaggc
     3721 agattgagtg gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt
     3781 attttgttga taatggtcag ctaattgaac agaaccatct tcaatgttgt gtctaatttt
     3841 gaagttaact ttgataccat tcttttgttt gtcagccatg atgtaaacat tgtgagagtt
     3901 atagttgtat tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt
     3961 taattcgatt ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta
     4021 gttaccgtca tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga
     4081 cttgaaaaag tcatgttgtt tcatatgatc tgggtatctc gcaaaacatt gaacaccata
     4141 accgaaagta gtgactaagg ttggccatgg aactggcaat ttaccagtag tacaaataaa
     4201 ttttaaggtc aatttaccgt aagtagcatc accttcacct tcaccggaga cagaaaattt
     4261 gtgaccatta acatcaccat ctaattcaac caaaattggg acaacaccag tgaataattc
     4321 ttcaccttta gacatagcct gcttttttgt acaaacttgt tgaatcgatt gtaaagtttg
     4381 ttgatgttaa ttgatttgaa ttcaattgtg atgaataaaa gaaagaaaaa gaaaaagaaa
     4441 aaaagaaaga agaaataaat aaattaaaaa aattttccaa ttgaagggaa tttcaagggt
     4501 taatatatat taagtcaaaa aaaatccaat tttcgttgga attggctttg acaagacaaa
     4561 ttttagtggt tgagtgggtt ggttgaaaca atttccacct atttttgtct attttggcta
     4621 tttttgcata atatgatttg tacgggacac gccatacccg gggcattatc atccattcag
     4681 aagcaatttt gcgtcattat ccattttact aactaactat taagcaaaga aaagaaaaaa
     4741 tcatcacaac aaaaataaaa aatgtgcacc acgagcctgg atttgaaaca gctgatccaa
     4801 aaaccgcaac ctaattaatt agacaatacc tttcagttaa caccgattat accttttatt
     4861 gaactcaaac tagcatttga tgatactgat ttattcaatt gatggtatta tatcagtcaa
     4921 ttgctctgaa agcaaaatat attcggataa ccgagtgttt agagaataag cttctacgta
     4981 aaataataaa ctgcatgaaa aaaattaagg gggttaaaga aattgccagc caaaatgtga
     5041 gtaaatttgt tataattcac ggctctcctt gtaatttttc atggaacact atcaattttt
     5101 cggaattgaa ccacgcgatt tgtattttgc aagtcaaatt aatcatactg aatactgtat
     5161 ggtgacgttc caaattgacc ctaaaataaa atatcgcaat tgtctgtatg tgtgtgctcg
     5221 agcgaagtag tcctcactga ggagatccca ggctagaaat cagcaaattt tacatccctc
     5281 atattctgac acgagtttaa ctacatagca attgtaaaag ggctcttgtt tttttttttt
     5341 ttttgtggcg gtgcagtacg tacacgatct ttaaagatta gggctataac catgtcaaag
     5401 tgactcattt tttttgttca aatgcgcact cggatttaaa gcagcaaaaa aatacttcgt
     5461 tacaaaaatg gaagcgcatt ttccgaaaaa ggttaattcc tagcgatttt attatttttg
     5521 ttttatttat taatttttct tgttttgcta ttgcattttt gcaaccaaaa atagaaaatt
     5581 ctcgtttttt tttgttgtag cgcaaccaat ttctggttat tctttttttt ttgcaatttt
     5641 taaatttgca cgtgacagtg ttagtgttag tgcaggcctg atatccccta tagtgagtcg
     5701 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     5761 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     5821 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     5881 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     5941 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     6001 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     6061 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     6121 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     6181 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     6241 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     6301 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     6361 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     6421 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     6481 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     6541 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     6601 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     6661 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     6721 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     6781 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6841 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6901 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6961 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7021 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7081 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7141 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7201 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7261 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7321 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7381 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7441 atgtt
//
