Basic Vector Information
- Vector Name:
- NORcp_Reporter
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4360 bp
- Type:
- Cloning vector
- Replication origin:
- pSC101 ori
- Source/Author:
- Groseclose TM, Rondon RE, Herde ZD, Aldrete CA
NORcp_Reporter vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
NORcp_Reporter vector Sequence
LOCUS 62056_1775 4360 bp DNA circular SYN 10-SEP-2020
DEFINITION Cloning vector NORcp_Reporter, complete sequence.
ACCESSION MT127261
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4360)
AUTHORS Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
TITLE Engineered systems of inducible anti-repressors for the next
generation of biological programming
JOURNAL Nat Commun 11 (1), 4440 (2020)
PUBMED 32895374
REFERENCE 2 (bases 1 to 4360)
AUTHORS Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
TITLE Direct Submission
JOURNAL
REFERENCE 3 (bases 1 to 4360)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(28-FEB-2020) Chemical "
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4360
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 51..56
/label=trc promoter -35 hexamer
/note="trc promoter -35 hexamer"
/regulatory_class="promoter"
regulatory 57..73
/label=operator Oagg
/note="operator Oagg"
/regulatory_class="other"
regulatory 74..79
/label=trc promoter -10 hexamer
/note="trc promoter -10 hexamer"
/regulatory_class="promoter"
regulatory 95..114
/label=operator Ottg
/note="operator Ottg"
/regulatory_class="other"
regulatory 131..204
/label=RiboJ10
/note="RiboJ10"
/regulatory_class="insulator"
regulatory 223..234
/label=strong bacterial RBS
/note="strong bacterial RBS"
/regulatory_class="ribosome_binding_site"
CDS 241..954
/label=superfolder GFP
/note="GFP variant that folds robustly even when fused to
poorly folded proteins (Nager et al., 2011)"
terminator 969..1055
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(1596..2543)
/label=Rep101
/note="RepA protein needed for replication with the pSC101
origin"
rep_origin complement(2591..2813)
/direction=LEFT
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
terminator complement(3305..3399)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(3433..4224)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
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