LOCUS       62056_1775        4360 bp DNA     circular SYN 10-SEP-2020
DEFINITION  Cloning vector NORcp_Reporter, complete sequence.
ACCESSION   MT127261
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next 
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
  PUBMED    32895374
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4360)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2020) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      51..56
                     /label=trc promoter -35 hexamer
                     /note="trc promoter -35 hexamer"
                     /regulatory_class="promoter"
     regulatory      57..73
                     /label=operator Oagg
                     /note="operator Oagg"
                     /regulatory_class="other"
     regulatory      74..79
                     /label=trc promoter -10 hexamer
                     /note="trc promoter -10 hexamer"
                     /regulatory_class="promoter"
     regulatory      95..114
                     /label=operator Ottg
                     /note="operator Ottg"
                     /regulatory_class="other"
     regulatory      131..204
                     /label=RiboJ10
                     /note="RiboJ10"
                     /regulatory_class="insulator"
     regulatory      223..234
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             241..954
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      969..1055
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1596..2543)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2591..2813)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3305..3399)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3433..4224)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtccaac atatggcacc ggaggctttc gtcttcacct cgagaattgt ttgacattag
       61 gagcgctcct aattataatt cgtgcacttc gtgcaatttt gagcgctcaa aattggactg
      121 cttgcacctg agcgctcaac gggtgtgctt cccgttctga tgagtccgtg aggacgaaag
      181 cgcctctaca aataatttgt ttaactgagc acagaattca ttaaagagga gaaaggtacc
      241 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      301 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
      361 aagcttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      421 gtcactactc tcacttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg
      481 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      541 aaagatgacg ggacttacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      601 aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa
      661 cttgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
      721 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gatccgttca actagcagac
      781 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      841 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
      901 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaataatct
      961 agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1021 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagacc tagggtacgg
     1081 gttttgctgc ccgcaaacgg gctgttctgg tgttgctagt ttgttatcag aatcgcagat
     1141 ccggcttcag ccggtttgcc ggctgaaagc gctatttctt ccagaattgc catgattttt
     1201 tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg ataagcagca
     1261 tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt
     1321 caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta ggtgttacat
     1381 gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttaaat gcaccaaaaa
     1441 ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg catatggaca
     1501 gttttccctt tgatatctaa cggtgaacag ttgttctact tttgtttgtt agtcttgatg
     1561 cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt agccagtatg
     1621 ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatgc
     1681 ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt
     1741 aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga tgtaatggtt
     1801 gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc
     1861 atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca
     1921 accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat
     1981 tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca
     2041 tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac
     2101 cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat
     2161 tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct
     2221 aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc
     2281 aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca
     2341 ccataagcat tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat
     2401 accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat
     2461 aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct
     2521 ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac
     2581 caattgagat gggctagtca atgataatta ctagtccttt tcccgggaga tctgggtatc
     2641 tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat
     2701 tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata
     2761 aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt
     2821 tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac
     2881 agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat
     2941 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
     3001 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg
     3061 gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg
     3121 ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc
     3181 ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta
     3241 atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg tccctagtgc
     3301 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     3361 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc tcgaacccca
     3421 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     3481 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     3541 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     3601 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     3661 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     3721 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     3781 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     3841 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     3901 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     3961 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     4021 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     4081 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     4141 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     4201 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct
     4261 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     4321 cttcccaacc ttaccagagg gcgccccagc tggcaattcc
//
