6-AXIG-WUS2-IN2 vector (V015861)

MSDS

Basic Vector Information

Vector Name:: 6-AXIG-WUS2-IN2

Antibiotic Resistance:: Kanamycin

Length:: 5611 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Gao H.

6-AXIG-WUS2-IN2 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

6-AXIG-WUS2-IN2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_181        5611 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 6-AXIG:WUS2:IN2, complete sequence.
ACCESSION   MN294718
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(274..301)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(393..479)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    519..614
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      663..1973
                     /regulatory_class="promoter"
     CDS             1975..2883
                     /codon_start=1
                     /transl_table=11
                     /product="ZM-WUS2"
                     /label=ZM-WUS2
                     /protein_id="QIN53505.1"
                     /translation="MAANAGGGGAGGGSGSGSVAAPAVCRPSGSRWTPTPEQIRMLKEL
                     YYGCGIRSPSSEQIQRITAMLRQHGKIEGKNVFYWFQNHKARERQKRRLTSLDVNVPAA
                     GAADATTSQLGVLSLSSPPPSGAAPPSPTLGFYAAGNGGGSAVLLDTSSDWGSSGAAMA
                     TETCFLQDYMGVTDTGSSSQWPRFSSSDTIMAAAAARAATTRAPETLPLFPTCGDDGGS
                     GSSSYLPFWGAASTTAGATSSVAIQQQHQLQEQYSFYSNSNSTQLAGTGNQDVSATAAA
                     AAALELSLSSWCSPYPAAGSM"
     regulatory      2959..3453
                     /regulatory_class="terminator"
     primer_bind     complement(3608..3624)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3662..3786
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3859..3877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3882..3898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4011..4817
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4967..5555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

This page is informational only.