LOCUS       62056_181        5611 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 6-AXIG:WUS2:IN2, complete sequence.
ACCESSION   MN294718
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(274..301)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(393..479)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    519..614
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      663..1973
                     /regulatory_class="promoter"
     CDS             1975..2883
                     /codon_start=1
                     /transl_table=11
                     /product="ZM-WUS2"
                     /label=ZM-WUS2
                     /protein_id="QIN53505.1"
                     /translation="MAANAGGGGAGGGSGSGSVAAPAVCRPSGSRWTPTPEQIRMLKEL
                     YYGCGIRSPSSEQIQRITAMLRQHGKIEGKNVFYWFQNHKARERQKRRLTSLDVNVPAA
                     GAADATTSQLGVLSLSSPPPSGAAPPSPTLGFYAAGNGGGSAVLLDTSSDWGSSGAAMA
                     TETCFLQDYMGVTDTGSSSQWPRFSSSDTIMAAAAARAATTRAPETLPLFPTCGDDGGS
                     GSSSYLPFWGAASTTAGATSSVAIQQQHQLQEQYSFYSNSNSTQLAGTGNQDVSATAAA
                     AAALELSLSSWCSPYPAAGSM"
     regulatory      2959..3453
                     /regulatory_class="terminator"
     primer_bind     complement(3608..3624)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3662..3786
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3859..3877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3882..3898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4011..4817
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4967..5555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
      241 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      301 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
      361 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
      421 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga
      481 tgcctggcag ttccctactc tcgcgttcat ggagctccaa ataatgattt tattttgact
      541 gatagtgacc tgttcgttgc aacaaattga taagcaatgc ttttttataa tgccaacttt
      601 gtatagaaaa gttgggccga gctcggtacg gccagaatgg cccggaccgg gttaccgaat
      661 tcaggcgacc catcgctgct ttgtctacat catgttcttc atcatcctcc ccaggcgacg
      721 cgtgctgctg ttcttattca gactaccgtt cgagtgactg catggcgtac atctttctgc
      781 atcgactttg tacggctaca tcgaacatat acacgagatg tctcgtgtga atagagtcac
      841 taatgcctta agcatcggtt actccgtagg gtacattctg ttcttcttat ttgtgcatat
      901 ttttattgtt gtttactgat tatacgagta gttatacata catgcacata catatcatca
      961 catatatcac aatatttttc taaattaaat taaaactaaa aatgactaaa tttctaacac
     1021 caacgacatt gtaatgtttt ctccaacaac tttacctatt ctacattgtt ctatttcgaa
     1081 tttcactcta taaacaacat agtctacaat ggaaaacagt gctttgtacg actatatacg
     1141 cgatgtgtgg ctacaacata agacaatata gtcgtttgaa gattgaacct atatatcggt
     1201 acggttaatc cgtctatgta cgtgggcatg acgaacaccc gtgataacga aggattaacg
     1261 tgcacaatca taaatccaaa gtaggagcgg tgcatgatga gaatcgctct cagtactcga
     1321 cataatgaac cttacgaggt acaacaggca ggcaggcagg gaccaggggc cgcctttatt
     1381 tcaggctcgc tggccccacg ggcgtgctgc gtgcacgaag ggcactaccc caacctctca
     1441 ccgaaaaccg cgctggatcg gcaaatcaaa cgaggtggtg ccccgtgccc actctccacg
     1501 tccacggcac catccctctg cagccgctca ccagccatgc cgtgtcgcgg aacggcacaa
     1561 ccacccccaa cccactcacg aaaccccgtc ccggccgtgc ccgtgtcggt ccgcgctcgg
     1621 caacgaggcg gcccgcgctg ctgagtcccc tggacacccg acaccctgtc ggccctttgt
     1681 ttattcatcc cgaaatctca tctgccccca cggccgactg cgctgcgccg cccggatata
     1741 tatacccatc gttatcgatc gatcgatcgc gtcactcacg ggtagctcat ggtcgagcgt
     1801 agcatgcagg aacttatttg ccgtgcgctc ccaggtctcc gctcgcgtgc cttccagtct
     1861 gtctcacact agctgctgtg ggacgatcga agtgggtgtg tcagctagct agctgcgccg
     1921 tgaccacgca catgaccgca gtgcgcgcgg ggctgatcaa gggaaagtga tcccatggcg
     1981 gccaatgcgg gcggcggtgg agcgggagga ggcagcggca gcggcagcgt ggctgcgccg
     2041 gcggtgtgcc gccccagcgg ctcgcggtgg acgccgacgc cggagcagat caggatgctg
     2101 aaggagctct actacggctg cggcatccgg tcgcccagct cggagcagat ccagcgcatc
     2161 accgccatgc tgcggcagca cggcaagatc gagggcaaga acgtcttcta ctggttccag
     2221 aaccacaagg cccgcgagcg ccagaagcgc cgcctcacca gcctcgacgt caacgtgccc
     2281 gccgccggcg cggccgacgc caccaccagc caactcggcg tcctctcgct gtcgtcgccg
     2341 ccgccttcag gcgcggcgcc tccctcgccc accctcggct tctacgccgc cggcaatggc
     2401 ggcggatcgg ctgtgctgct ggacacgagt tccgactggg gcagcagcgg cgctgctatg
     2461 gccaccgaga catgcttcct gcaggactac atgggcgtga cggacacggg cagctcgtcg
     2521 cagtggccac gcttctcgtc gtcggacacg ataatggcgg cggccgcggc gcgggcggcg
     2581 acgacgcggg cgcccgagac gctccctctc ttcccgacct gcggcgacga cggcggcagc
     2641 ggtagcagca gctacttgcc gttctggggt gccgcgtcca caactgccgg cgccacttct
     2701 tccgttgcga tccaacagca acaccagctg caggagcagt acagctttta cagcaacagc
     2761 aacagcaccc agctggccgg caccggcaac caagacgtat cggcaacagc agcagcagcc
     2821 gccgccctgg agctgagcct cagctcatgg tgctcccctt accctgctgc agggagtatg
     2881 tgagagcaac gcgagctgcc actgctcttc actgatgtct ctggaatgga aggaggagga
     2941 agtgagaaca gatcaattcg acaaagcagc attagtccgt tgatcggtgg aagaccactc
     3001 gtcagtgttg agttgaatgt ttgatcaata aaatacggca atgctgtaag ggttgttttt
     3061 tatgccattg ataatacact gtactgttca gttgttgaac tctatttctt agccatgcca
     3121 agtgcttttc ttattttgaa taacattaca gcaaaaagtt gaaagacaaa aaaaaaaacc
     3181 cccgaacaga gtgctttggg tcccaagctt ctttagactg tgttcggcgt tccccctaaa
     3241 tttctccccc tatatctcac tcacttgtca catcagcgtt ctctttcccc ctatatctcc
     3301 acgctctaca gcagttccac ctatatcaaa cctctatacc ccaccacaac aatattatat
     3361 actttcatct tcaactaact catgtacctt ccaatttttt tctactaata attatttacg
     3421 tgcacagaaa cttagcaagg agagagagag cggggtgacc caccttgcta gttggatatt
     3481 acctcttctc ttcaaagtat ccttgaacgc tcaccggtta tcaaatctct acactatagc
     3541 tctgtagtct tgctagatag ttagttcttt agctctcggt gaccaagctt ggcgcgatca
     3601 agcttatcga taccgtcgac ctcgaagctt ggtcacccgg tccgggccta gaaggccagc
     3661 ttcaagtttg tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt
     3721 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc
     3781 actatgaatc aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc
     3841 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     3901 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     3961 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     4021 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     4081 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     4141 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     4201 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     4261 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     4321 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     4381 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     4441 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     4501 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     4561 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     4621 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     4681 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     4741 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     4801 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     4861 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     4921 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4981 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5041 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5101 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5161 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5221 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5281 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5341 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5401 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5461 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5521 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5581 ggttcctggc cttttgctgg ccttttgctc a
//
