Basic Vector Information
- Vector Name:
- AbVec6W-hIgKappa
- Antibiotic Resistance:
- Streptomycin
- Length:
- 6338 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Davis CW, Jackson KJL., McElroy AK, Halfmann P
- Promoter:
- EM7
AbVec6W-hIgKappa vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AbVec6W-hIgKappa vector Sequence
LOCUS 62056_271 6338 bp DNA circular SYN 17-JUN-2019
DEFINITION Cloning vector AbVec6W-hIgKappa, complete sequence.
ACCESSION MK909075
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6338)
AUTHORS Davis CW, Jackson KJL., McElroy AK, Halfmann P, Huang J, Chennareddy
C, Piper AE, Leung Y, Albarino CG, Crozier I, Ellebedy AH, Sidney J,
Sette A, Yu T, Nielsen SCA., Goff AJ, Spiropoulou CF, Saphire EO,
Cavet G, Kawaoka Y, Mehta AK, Glass PJ, Boyd SD, Ahmed R.
TITLE Longitudinal Analysis of the Human B Cell Response to Ebola Virus
Infection
JOURNAL Cell 177 (6), 1566-1582 (2019)
PUBMED 31104840
REFERENCE 2 (bases 1 to 6338)
AUTHORS Davis CW, Ahmed R.
TITLE Direct Submission
JOURNAL Submitted (09-MAY-2019) Microbiology and Immunology, Emory
University, 1462 Clifton Rd, Atlanta, GA 30322, USA
REFERENCE 3 (bases 1 to 6338)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
date: "2019"; volume: "177"; issue: "6"; pages: "1566-1582"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-MAY-2019) Microbiology and Immunology, Emory University, 1462
Clifton Rd, Atlanta, GA 30322, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6338
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 384..587
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 818..836
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
misc_feature 961..966
/gene="IgK"
/label=AgeI site for VK cloning
/note="AgeI site for VK cloning"
V_region 976..1311
/gene="IgK"
/note="VK from Ebola virus glycoprotein-specific mAb
9.6.3A06"
CDS 1315..1626
/label=hIg-kappa-CL
/note="Human immunoglobulin kappa light chain constant
region"
misc_feature 1647..2234
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 2341..2389
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter complement(2560..2578)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2583..2599)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2667..3095
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3109..3438
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 3486..3533
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 3552..3947
/label=BSD
/note="blasticidin S deaminase"
polyA_signal 4108..4241
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4435..5023)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5122..5910)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
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