AbVec6W-hIgKappa vector (V015830)

LOCUS       62056_271        6338 bp DNA     circular SYN 17-JUN-2019
DEFINITION  Cloning vector AbVec6W-hIgKappa, complete sequence.
ACCESSION   MK909075
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Davis CW, Jackson KJL., McElroy AK, Halfmann P, Huang J, Chennareddy
            C, Piper AE, Leung Y, Albarino CG, Crozier I, Ellebedy AH, Sidney J,
            Sette A, Yu T, Nielsen SCA., Goff AJ, Spiropoulou CF, Saphire EO, 
            Cavet G, Kawaoka Y, Mehta AK, Glass PJ, Boyd SD, Ahmed R.
  TITLE     Longitudinal Analysis of the Human B Cell Response to Ebola Virus 
            Infection
  JOURNAL   Cell 177 (6), 1566-1582 (2019)
  PUBMED    31104840
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Davis CW, Ahmed R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Microbiology and Immunology, Emory 
            University, 1462 Clifton Rd, Atlanta, GA 30322, USA
REFERENCE   3  (bases 1 to 6338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2019"; volume: "177"; issue: "6"; pages: "1566-1582"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2019) Microbiology and Immunology, Emory University, 1462 
            Clifton Rd, Atlanta, GA 30322, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        818..836
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    961..966
                     /gene="IgK"
                     /label=AgeI site for VK cloning
                     /note="AgeI site for VK cloning"
     V_region        976..1311
                     /gene="IgK"
                     /note="VK from Ebola virus glycoprotein-specific mAb
                     9.6.3A06"
     CDS             1315..1626
                     /label=hIg-kappa-CL
                     /note="Human immunoglobulin kappa light chain constant
                     region"
     misc_feature    1647..2234
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    2341..2389
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(2560..2578)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2583..2599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2667..3095
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3109..3438
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3486..3533
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3552..3947
                     /label=BSD
                     /note="blasticidin S deaminase"
     polyA_signal    4108..4241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4435..5023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5122..5910)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"