LOCUS       62056_271        6338 bp DNA     circular SYN 17-JUN-2019
DEFINITION  Cloning vector AbVec6W-hIgKappa, complete sequence.
ACCESSION   MK909075
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Davis CW, Jackson KJL., McElroy AK, Halfmann P, Huang J, Chennareddy
            C, Piper AE, Leung Y, Albarino CG, Crozier I, Ellebedy AH, Sidney J,
            Sette A, Yu T, Nielsen SCA., Goff AJ, Spiropoulou CF, Saphire EO, 
            Cavet G, Kawaoka Y, Mehta AK, Glass PJ, Boyd SD, Ahmed R.
  TITLE     Longitudinal Analysis of the Human B Cell Response to Ebola Virus 
            Infection
  JOURNAL   Cell 177 (6), 1566-1582 (2019)
  PUBMED    31104840
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Davis CW, Ahmed R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Microbiology and Immunology, Emory 
            University, 1462 Clifton Rd, Atlanta, GA 30322, USA
REFERENCE   3  (bases 1 to 6338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2019"; volume: "177"; issue: "6"; pages: "1566-1582"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2019) Microbiology and Immunology, Emory University, 1462 
            Clifton Rd, Atlanta, GA 30322, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        818..836
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    961..966
                     /gene="IgK"
                     /label=AgeI site for VK cloning
                     /note="AgeI site for VK cloning"
     V_region        976..1311
                     /gene="IgK"
                     /note="VK from Ebola virus glycoprotein-specific mAb
                     9.6.3A06"
     CDS             1315..1626
                     /label=hIg-kappa-CL
                     /note="Human immunoglobulin kappa light chain constant
                     region"
     misc_feature    1647..2234
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    2341..2389
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(2560..2578)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2583..2599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2667..3095
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3109..3438
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3486..3533
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3552..3947
                     /label=BSD
                     /note="blasticidin S deaminase"
     polyA_signal    4108..4241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4435..5023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5122..5910)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
      661 gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg
      721 acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta gaacgcggct
      781 acaattaata cataacctta tgtatcatac acatacgatt taggtgacac tatagaataa
      841 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc acctcggttc
      901 tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt agcaactgca
      961 accggtgtac atggggatat tgtgatgacc cagactcccc tctcctcagc tgtcaccctt
     1021 ggacagccgg cctccatctc ctgcaggtct agtcaaaggc tcgtacacag tgatggaaac
     1081 acctacttga gttggcttca tcagaggcca ggccagcctc cacgactcct gatttataag
     1141 gtttctctcc gcttctctgg ggtcccagac agattcagtg gcagtggggc agggacagat
     1201 ttcacattga aaatcagtag ggttgaagct gaggatgtcg gaatttatta ctgcatgcaa
     1261 gctacccaat ttcctctcac tttcggcgga gggaccaagg tggagatcaa acgtacggtg
     1321 gctgcaccat ctgtcttcat cttcccgcca tctgatgagc agttgaaatc tggaactgcc
     1381 tctgttgtgt gcctgctgaa taacttctat cccagagagg ccaaagtaca gtggaaggtg
     1441 gataacgccc tccaatcggg taactcccag gagagtgtca cagagcagga cagcaaggac
     1501 agcacctaca gcctcagcag caccctgacg ctgagcaaag cagactacga gaaacacaaa
     1561 gtctacgcct gcgaagtcac ccatcagggc ctgagctcgc ccgtcacaaa gagcttcaac
     1621 agggggagag tgttagaagc ttgatcctca acctctggat tacaaaattt gtgaaagatt
     1681 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     1741 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     1801 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     1861 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     1921 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     1981 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2041 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2101 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2161 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2221 ggccgcctcc ccgcctggat cccgtacaaa gtggttgatc tagagggccc gcggttcgct
     2281 gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     2341 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     2401 gttcggtccc agggctggca ctctgtcgat accccaccgt gaccccattg gggccaatac
     2461 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     2521 gcagccaacg tcggggcggc aggccctgcc atagcatccc ctatagtgag tcgtattaca
     2581 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tggatctgcg
     2641 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     2701 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2761 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2821 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2881 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2941 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3001 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3061 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     3121 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3181 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3241 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3301 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3361 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3421 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     3481 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     3541 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     3601 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     3661 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     3721 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     3781 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     3841 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     3901 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg
     3961 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     4021 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4081 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     4141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4201 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgct cttccgctgc
     4261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4861 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5041 ttctacgggg tctgacgctc agtggaacga cgcgtaactc acgttaaggg attttggtca
     5101 tgggtggctc gacgagggtt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     5161 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     5221 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     5281 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     5341 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     5401 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     5461 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     5521 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     5581 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     5641 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     5701 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     5761 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     5821 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     5881 cgatacttcg gcgatcaccg cttccctcat aatgtttaac tttgttttag ggcgactgcc
     5941 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     6001 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat
     6061 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     6121 tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt
     6181 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggta gcagcgaagt
     6241 cgaggcattt ctgtcctggc tggtctagaa ttgcatgaag aatctgctta gggttaggcg
     6301 ttttgcgctg cttcgcgatg tacgggccag atatacgc
//
