pAY-FE vector (V015774)

LOCUS       62056_3305        5356 bp DNA     circular SYN 26-JUL-2021
DEFINITION  Cloning vector pAY-FE, complete sequence.
ACCESSION   MW464120
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Yee A, Dai M, Croteau SE, Shavit JA, Pipe SW, Siemieniak D, Meng F, 
            Ginsburg D.
  TITLE     Phage Display Broadly Identifies Inhibitor-reactive Regions in von 
            Willebrand Factor
  JOURNAL   J Thromb Haemost (2021) In press
  PUBMED    34255925
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Yee A, Dai M, Siemieniak D, Shavit JA, Pipe SW, Croteau SE, Meng F, 
            Ginsburg D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2021) Pediatrics, Baylor College of Medicine, 1102
            Bates Ave. Rm. C.1025.09, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 5356)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Thromb 
            Haemost (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JAN-2021) Pediatrics, Baylor College of Medicine, 1102 Bates 
            Ave. Rm. C.1025.09, Houston, TX 77030, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2269..2322
                     /label=TorT signaling sequence
                     /note="TorT signaling sequence"
     promoter        2359..2389
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2443..3099
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3444..3746
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             3808..3831
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    3841..3879
                     /label=E tag
                     /note="E tag"
     misc_feature    3889..4479
                     /label=truncated pIII
                     /note="truncated pIII"
     primer_bind     complement(4486..4502)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4715..5170
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"