LOCUS       62056_3305        5356 bp DNA     circular SYN 26-JUL-2021
DEFINITION  Cloning vector pAY-FE, complete sequence.
ACCESSION   MW464120
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Yee A, Dai M, Croteau SE, Shavit JA, Pipe SW, Siemieniak D, Meng F, 
            Ginsburg D.
  TITLE     Phage Display Broadly Identifies Inhibitor-reactive Regions in von 
            Willebrand Factor
  JOURNAL   J Thromb Haemost (2021) In press
  PUBMED    34255925
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Yee A, Dai M, Siemieniak D, Shavit JA, Pipe SW, Croteau SE, Meng F, 
            Ginsburg D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2021) Pediatrics, Baylor College of Medicine, 1102
            Bates Ave. Rm. C.1025.09, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 5356)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Thromb 
            Haemost (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JAN-2021) Pediatrics, Baylor College of Medicine, 1102 Bates 
            Ave. Rm. C.1025.09, Houston, TX 77030, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2269..2322
                     /label=TorT signaling sequence
                     /note="TorT signaling sequence"
     promoter        2359..2389
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2443..3099
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3444..3746
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             3808..3831
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    3841..3879
                     /label=E tag
                     /note="E tag"
     misc_feature    3889..4479
                     /label=truncated pIII
                     /note="truncated pIII"
     primer_bind     complement(4486..4502)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4715..5170
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgct cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcataca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt tggagccttt tttttggaga ttttcaacat gcgtgttctg
     2281 ctgttcctgc tgctgtctct gttcatgctg ccggctttct cggcctagcc ggccggcgcg
     2341 ccattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     2401 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     2461 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     2521 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     2581 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     2641 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     2701 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     2761 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     2821 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2881 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2941 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     3001 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     3061 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     3121 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     3181 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     3241 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     3301 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     3361 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     3421 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     3481 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     3541 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     3601 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtaccgg
     3661 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     3721 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     3781 caggtcgacg cggccgcagg tggtggtgac tacaaggacg atgacgataa aggaggagga
     3841 ggtgcgccgg tgccgtatcc ggatccgctg gaaccgcgtg ccgcacaggg cggcggctct
     3901 ggtggtggtt ctggtggcgg ctctgagggt ggcggctctg agggtggcgg ctctgagggt
     3961 ggcggctctg agggtggcgg ttctgagggt ggcggctctg agggtggcgg ttccggttcc
     4021 ggtgattttg attatgaaaa aatggcaaac gctaataagg gggctatgac cgaaaatgcc
     4081 gatgaaaacg cgctgcagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac
     4141 ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct
     4201 actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca
     4261 cctttaatga ataatttccg tcaatattta ccttctttgc ctcagtcggt tgaatgtcgc
     4321 ccttatgtct ttggcgctgg taaaccgtat gaattttcta ttgattgtga caaaataaac
     4381 ttattccgtg gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttcg
     4441 acgtttgcta acatactgcg taataaggag tcttaataag aattcactgg ccgtcgtttt
     4501 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4561 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4621 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4681 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     4741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4921 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5041 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5161 tttacaattt tatggtgcag tctcagtaca atctgctctg atgccgcata gttaagccag
     5221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5341 tcaccgaaac gcgcga
//
