pSB201 vector (V015768)

LOCUS       62056_19270        2989 bp DNA     circular SYN 17-MAR-2021
DEFINITION  Cloning vector pSB201, complete sequence.
ACCESSION   MW574253
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA.
  TITLE     Single-cell measurement of plasmid copy number and promoter activity
  JOURNAL   Nat Commun 12 (1), 1475 (2021)
  PUBMED    33674569
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2021) Biological Engineering, MIT, 500 Tech Sq 
            NE47-140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 2989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2021"; volume: "12"; issue: "1"; pages: "1475"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-FEB-2021) Biological Engineering, MIT, 500 Tech Sq NE47-140, 
            Cambridge, MA 02139, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        113..163
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             213..929
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      933..994
                     /regulatory_class="terminator"
     rep_origin      complement(1440..1985)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2077..2889)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"