LOCUS       62056_19270        2989 bp DNA     circular SYN 17-MAR-2021
DEFINITION  Cloning vector pSB201, complete sequence.
ACCESSION   MW574253
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA.
  TITLE     Single-cell measurement of plasmid copy number and promoter activity
  JOURNAL   Nat Commun 12 (1), 1475 (2021)
  PUBMED    33674569
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2021) Biological Engineering, MIT, 500 Tech Sq 
            NE47-140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 2989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2021"; volume: "12"; issue: "1"; pages: "1475"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-FEB-2021) Biological Engineering, MIT, 500 Tech Sq NE47-140, 
            Cambridge, MA 02139, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        113..163
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             213..929
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      933..994
                     /regulatory_class="terminator"
     rep_origin      complement(1440..1985)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2077..2889)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttgat
       61 aagtccctaa cttttacagc tagctcagtc ctaggtatta tgctagcctg aagctgtcac
      121 cggatgtgct ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt
      181 gtttaatact agagaaagag gggaaatact agatggtgag caagggcgag gagctgttca
      241 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      301 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      361 ccacaggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
      421 aatgcttcgc ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc
      481 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      541 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      601 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      661 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      721 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac accccaatcg
      781 gcgacggccc cgtgctgctg cccgacaacc actaccttag ctaccagtcc gccctgagca
      841 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      901 tcactctcgg catggacgag ctgtacaagt aactcggtac caaattccag aaaagaggcc
      961 tcccgaaagg ggggcctttt ttcgttttgg tccgatcctc tacgccggac gcatcgtggc
     1021 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1081 ggaagatcgg gctcgccact tcgggctcat gagcaaatat tttatctgag gtgcttcctc
     1141 gctcactgac tcgctgcacg aggcagacct cagcgctagc ggagtgtata ctggcttact
     1201 atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc
     1261 accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc
     1321 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
     1381 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt
     1441 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     1501 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
     1561 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     1621 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     1681 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1741 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     1801 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     1861 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
     1921 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
     1981 ctcaagaaga tcatcttatt aaggggtctg acgctcagtg gaacgaaaaa tcaatctaaa
     2041 gtatatatga gtaaacttgg tctgacagtt accttagaaa aactcatcga gcatcaaatg
     2101 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     2161 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     2221 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     2281 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
     2341 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     2401 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     2461 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     2521 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     2581 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     2641 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     2701 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     2761 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     2821 taaatcagca tccatgtggg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     2881 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     2941 tgatatattt ttatcttgtg caatgtacat cagagatttt gagacacaa
//
