pCC-TM52 vector (V015766)

LOCUS       V015766                 4410 bp    DNA     circular SYN 30-JUL-2019
DEFINITION  Exported.
ACCESSION   V015766
VERSION     V015766
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Engineering DNA recognition and allosteric response properties of
            TetR family proteins by using a module-swapping strategy
  JOURNAL   Nucleic Acids Res. (2019) In press
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2018) Department of Biology, The University of
            Texas at Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA
REFERENCE   3  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2018) Department of Biology, The University of Texas at
            Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA"
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..916
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1093..1681
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      1941..2027
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(2330..2351)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      2387..2428
                     /label="bidir terminator"
                     /note="bidir terminator"
                     /regulatory_class="terminator"
     regulatory      2446..2589
                     /regulatory_class="promoter"
     CDS             2610..3185
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-MphR"
                     /label="TetR-MphR"
                     /protein_id="QBZ38513.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYLISW
                     YELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDGELA
                     DHVLAQIAAILCLMFPEHDDFQLLQPHA"
     terminator      complement(3200..3294)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3335..4066)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        complement(4067..4171)
                     /label="AmpR promoter"
     terminator      4325..4372
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"