LOCUS       V015766                 4410 bp    DNA     circular SYN 30-JUL-2019
DEFINITION  Exported.
ACCESSION   V015766
VERSION     V015766
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Engineering DNA recognition and allosteric response properties of
            TetR family proteins by using a module-swapping strategy
  JOURNAL   Nucleic Acids Res. (2019) In press
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2018) Department of Biology, The University of
            Texas at Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA
REFERENCE   3  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2018) Department of Biology, The University of Texas at
            Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA"
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..916
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1093..1681
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      1941..2027
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(2330..2351)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      2387..2428
                     /label="bidir terminator"
                     /note="bidir terminator"
                     /regulatory_class="terminator"
     regulatory      2446..2589
                     /regulatory_class="promoter"
     CDS             2610..3185
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-MphR"
                     /label="TetR-MphR"
                     /protein_id="QBZ38513.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYLISW
                     YELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDGELA
                     DHVLAQIAAILCLMFPEHDDFQLLQPHA"
     terminator      complement(3200..3294)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3335..4066)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        complement(4067..4171)
                     /label="AmpR promoter"
     terminator      4325..4372
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaat aactgtcaga ccaagtttac tcatatatac tttagattga
      961 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1021 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1201 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     1261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcatgg
     1741 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     1801 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     1861 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     1921 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1981 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     2041 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     2101 ccaggcatca aattaagcag aaggccatcc gtcaggatgg cctttttgcg tttctacaaa
     2161 ctctgctagc aagtaaggcc gataagatgc atgccagttc taactctgct agcaagtaag
     2221 gccgataaga tgcatgccag ttctgctagc aagtaaggcc gataagatgc atgccactct
     2281 gctagcaagt aaggccgata agatgcatgc cagttctagc ataaccctaa tgagtgagct
     2341 aacttacatt aattgcgttg cgccttaatt aacggcactc ctcagcaaat ataatgaccc
     2401 tcttgataac ccaagagggc attttttaat gcccatggcg tttaccacag ctaacaccac
     2461 gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt acgggcatgc
     2521 ataaggctcg taggctatat tcagggagac cacaacggtt tccctctaca aataattttg
     2581 tttaactttg aaataaggag gtaatacaaa tgtctagatt agataaaagt aaagtgatta
     2641 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg
     2701 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
     2761 tgctcgttcg tatgatggaa cgtggtgttg aacaggttcg tcactacctg aacgcgatcc
     2821 cgatcggtgc gggtccgcag ggtctgtggg aattcctgca ggttctggtt cgttctatga
     2881 acacccgtaa cgacttctct gttaactacc tgatctcttg gtacgaactg caggttccgg
     2941 aactgcgtac cctggcgatc cagcgtaacc gtgcggttgt tgaaggtatc cgtaaacgtc
     3001 tgccgccggg tgcgccggcg gcggcggaac tgctgctgca ctctgttatc gcgggtgcga
     3061 ccatgcagtg ggcggttgac ccggacggtg aactggcgga ccacgttctg gcgcagatcg
     3121 cggcgatcct gtgcctgatg ttcccggaac acgacgactt ccagctgctg cagccgcacg
     3181 cgtaataact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     3241 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     3301 agctcgtaaa cttggtctga cagcccttaa cttacttatt aaataattta tagctattga
     3361 aaagagataa gaattgttca aagctaatat tgtttaaatc gtcaattcct gcatgtttta
     3421 aggaattgtt aaattgattt tttgtaaata ttttcttgta ttctttgtta acccatttca
     3481 taacgaaata attatacttt tgtttatctt tgtgtgatat tcttgatttt tttctactta
     3541 atctgataag tgagctattc actttaggtt taggatgaaa atattctctt ggaaccatac
     3601 ttaatataga aatatcaact tctgccatta aaagtaatgc caatgagcgt tttgtattta
     3661 ataatctttt agcaaacccg tattccacga ttaaataaat ctcattagct atactatcaa
     3721 aaacaatttt gcgtattata tccgtactta tgttataagg tatattacca tatattttat
     3781 aggattggtt tttaggaaat ttaaactgca atatatcctt gtttaaaact tggaaattat
     3841 cgtgatcaac aagtttattt tctgtagttt tgcataattt atggtctatt tcaatggcag
     3901 ttacgaaatt acacctcttt actaattcaa gggtaaaatg gccttttcct gagccgattt
     3961 caaagatatt atcatgttca tttaatctta tatttgtcat tattttatct atattatgtt
     4021 ttgaagtaat aaagttttga ctgtgtttta tatttttctc gttcatactc ttcctttttc
     4081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4201 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4261 ttcgtcttca ctaagcgact aaaaaattga atgtaggaaa ccaacatgcc agttcgagca
     4321 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc
     4381 tcaggcattt gagaagcaca cggtcacact
//
