Basic Vector Information
- Vector Name:
- pMAJIc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6674 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Pena MM, Facincani AP, Ferreira H, Ferro JA.
- Promoter:
- araBAD
pMAJIc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAJIc vector Sequence
LOCUS 62056_16470 6674 bp DNA circular SYN 29-JUN-2020 DEFINITION Cloning vector pMAJIc, complete sequence. ACCESSION MT119764 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6674) AUTHORS Pena MM, Facincani AP, Ferreira H, Ferro JA. TITLE Direct Submission JOURNAL Submitted (26-FEB-2020) Departamento de Tecnologia, Univ. Estadual Paulista 'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de Acesso Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil REFERENCE 2 (bases 1 to 6674) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (26-FEB-2020) Departamento de Tecnologia, Univ. Estadual Paulista 'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de Acesso Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil" FEATURES Location/Qualifiers source 1..6674 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 6..713 /label=mCherry /note="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" misc_feature 753..1561 /label=Xac_amy /note="Xac_amy" primer_bind complement(1577..1593) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 1734..2162 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS 2506..3297 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" CDS 3318..4175 /label=AmpR /note="beta-lactamase" rep_origin 4349..4937 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 5225..5246 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 5261..5291 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5299..5315 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5323..5339 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS complement(5447..6322) /label=araC /note="L-arabinose regulatory protein" promoter 6349..6633 /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" regulatory 6654..6659 /label=ribosome_binding_site /note="ribosome_binding_site" /regulatory_class="ribosome_binding_site"
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