LOCUS       62056_16470        6674 bp DNA     circular SYN 29-JUN-2020
DEFINITION  Cloning vector pMAJIc, complete sequence.
ACCESSION   MT119764
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Pena MM, Facincani AP, Ferreira H, Ferro JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Departamento de Tecnologia, Univ. Estadual 
            Paulista 'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de 
            Acesso Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, 
            Brazil
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Departamento de Tecnologia, Univ. Estadual Paulista 
            'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de Acesso 
            Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil"
FEATURES             Location/Qualifiers
     source          1..6674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..713
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    753..1561
                     /label=Xac_amy
                     /note="Xac_amy"
     primer_bind     complement(1577..1593)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1734..2162
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2506..3297
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             3318..4175
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4349..4937
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5225..5246
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5261..5291
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5299..5315
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5323..5339
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5447..6322)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        6349..6633
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      6654..6659
                     /label=ribosome_binding_site
                     /note="ribosome_binding_site"
                     /regulatory_class="ribosome_binding_site"
ORIGIN
        1 tcgagatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
       61 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      121 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      181 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      241 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      301 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      361 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      421 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      481 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
      541 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      601 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      661 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggaagcag
      721 gcggccgcgc actgaatcta gaatgacccg ggagctgctg catgctggcg ccaatggcga
      781 acgcgttgcg caccgcattg aacagcggaa agtcgtaggc cgcatgcggc gtggattgca
      841 ggtacggcgc caggaactgg tcgtaatcgc cattgcccgt accgccgccg gtgatcactt
      901 cgccgaagac atacacaccc gatcggatgc cggcatcgaa gacgcgattg agatggtcga
      961 acgtcatgtg cttggccgca tccacccgga agccggtcac gcccatgctc ttgagcgcct
     1021 gcagataggc gcgctgctgc tgtaccaccc agtcgttgcc cagcaaatcc ggcaggcctg
     1081 gatcgctgcc gccgccgcag atacggtaat tgcgcacctg gtaagcgtcg ttgtagttgc
     1141 tgatgcactg cgccgggccg aaatcgctgg cgctgaggaa gtttgactgc agcgtgccga
     1201 acaagcgcag cgaatcgtaa cggccggggt ttgccgcgta ctgcgacagg accgcgccgc
     1261 ctgggtaatt gaggtccgag cgtgtggccg cttcgttggc catgtggttg aacacgatgt
     1321 cggcataggt ttccacgccg ttgctggcca gcgcctggac catcttcttg aatgcagcgg
     1381 tgtcgccgag cggattgtcg atcaggcgga tgtcctgggg ttgatagcgc gcccaccagg
     1441 cacttccttc ggagcggtac gcgggggcga ccaatacctt gcgatagccg gcatcggcaa
     1501 tctgcttggc gcgcgcttcc acggtggcat acggccagtt gaaggcgtgc aggatgacat
     1561 ccccgggaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1621 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     1681 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     1741 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1801 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1861 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1921 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     1981 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2041 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2101 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2161 ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
     2221 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
     2281 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
     2341 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     2401 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     2461 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2521 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     2581 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     2641 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     2701 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     2761 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatcc
     2821 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     2881 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     2941 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3001 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     3061 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3121 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3181 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3241 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3301 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3361 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3421 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3481 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3541 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3601 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3661 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3721 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3781 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3841 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3901 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3961 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4021 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4081 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4141 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4201 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4261 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4321 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4381 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4441 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4501 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4561 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4621 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4681 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4741 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4801 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4861 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4921 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4981 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5041 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5101 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5161 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5221 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5281 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5341 atgattacga attcgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     5401 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     5461 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     5521 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     5581 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     5641 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     5701 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     5761 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     5821 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     5881 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     5941 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     6001 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     6061 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     6121 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     6181 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     6241 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     6301 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     6361 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     6421 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     6481 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     6541 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     6601 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agcaggagga
     6661 aagatccatg gcac
//
