pUWL201 vector (V015751)

LOCUS       V015751                 6780 bp    DNA     circular SYN 20-APR-2020
DEFINITION  Exported.
ACCESSION   V015751
VERSION     V015751
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Brown K, Nybo SE.
  TITLE     Next-generation sequencing of pIJ101-based Streptomyces-E. coli
            shuttle vectors
  JOURNAL   bioRxivorg (2020) In press
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Brown KV, Nybo SE.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2020) Department of Pharmaceutical Sciences,
            Ferris State University, 220 Ferris Drive, Big Rapids, MI 49307, USA
REFERENCE   3  (bases 1 to 6780)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. 1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JAN-2020) Department of Pharmaceutical Sciences, Ferris State
            University, 220 Ferris Drive, Big Rapids, MI 49307, USA"
FEATURES             Location/Qualifiers
     source          1..6780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     508..524
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      complement(633..671)
                     /label="derived from ermE*p"
                     /note="derived from ermE*p"
                     /regulatory_class="promoter"
     rep_origin      complement(1052..2422)
                     /direction=LEFT
                     /label="derived from pIJ101"
                     /note="derived from pIJ101"
     CDS             3564..4370
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     terminator      4569..4617
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      complement(4841..5296)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5323..5427
                     /label="AmpR promoter"
     CDS             5428..6285
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6459..6780
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"