LOCUS       V015751                 6780 bp    DNA     circular SYN 20-APR-2020
DEFINITION  Exported.
ACCESSION   V015751
VERSION     V015751
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Brown K, Nybo SE.
  TITLE     Next-generation sequencing of pIJ101-based Streptomyces-E. coli
            shuttle vectors
  JOURNAL   bioRxivorg (2020) In press
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Brown KV, Nybo SE.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2020) Department of Pharmaceutical Sciences,
            Ferris State University, 220 Ferris Drive, Big Rapids, MI 49307, USA
REFERENCE   3  (bases 1 to 6780)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. 1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JAN-2020) Department of Pharmaceutical Sciences, Ferris State
            University, 220 Ferris Drive, Big Rapids, MI 49307, USA"
FEATURES             Location/Qualifiers
     source          1..6780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     508..524
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      complement(633..671)
                     /label="derived from ermE*p"
                     /note="derived from ermE*p"
                     /regulatory_class="promoter"
     rep_origin      complement(1052..2422)
                     /direction=LEFT
                     /label="derived from pIJ101"
                     /note="derived from pIJ101"
     CDS             3564..4370
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     terminator      4569..4617
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      complement(4841..5296)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5323..5427
                     /label="AmpR promoter"
     CDS             5428..6285
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6459..6780
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      481 cgttggccga ttcattaatg cagagcttct agaactagtg gatcccccgg gctgcaggaa
      541 ttcgatatca agcttatcga taccgtcatg ctagccatat gtaatttctc ctctttaatg
      601 ttaaacaaaa ttatttctag aactagtgga tcgatcctac caaccggcac gattgtgccc
      661 acaacagcat cgcggtgcca cgtgtggacc gcgtcggtca gatcctcccc gcacctctcg
      721 ccagccgtca agatcgaccg cgtgcacctg cgatcgccga tcaaccgcga ctagcatcgg
      781 gcgcaagccg ccactcgaac ggacactcgc atggacgtcc ccttcctgga cctgcaagcc
      841 gcgtacctcg aactccggtc cgacatcgac caggcgtgcc ggcgcgtgct cgggtcgggc
      901 tggtaccgcc tgcacgaagt gccggcgggg ccgaccccgg gcgagtaatc ccaggattac
      961 tcccgcggct tcgaccccgg ccgccgtcgc cgcgtacgtc accgaccccc gccgtacgtc
     1021 accgggatga cgtacggcgg gggggagcga gttagtgcga agtgggccca cttgcgagcc
     1081 gggcgatgtg ccgggcggcc cgctcctggc ggtcgtcggc gtcgtcgtcc tggtcgtcgt
     1141 cctgctctcg ccgtcggcgt gcagttgctt cctcgcggcg ctgggcgagg gcggcgagca
     1201 tgtcggcgta cgcctcggcc acctcccccg ccgtgagcac caccactgtg tcggccgcgt
     1261 cggccagcgc caggacctcc cgcacccgtt cgcccacggc cgccgaatcc tcgttgccgt
     1321 ccttgccttc ggcggcccgg gtcgcctcga ggtcgagggc gcggcgggtg accgcgtgcc
     1381 atccgtcctc ggtcacggcg accccggccc gcagctcccc gccgtcggcg tcggccgcca
     1441 ggagcagatc gaggtcgtcg gcctcggtgt cgccgccgtc gagcccgagc atctgccgca
     1501 ggtagcgggt ccattcgatg gcccggcgtc cccgggttgc ccgctcgtac tcgtgccagc
     1561 gcgagaggtt ccactccagc gagccgaccc cggcggcgtc gtcctcggtc atgccgccgg
     1621 tcaggtcccc gatccgtccg aggagttcga acggggcgac gttcccgccg gtcgccgtct
     1681 tgaggtcggc gcgggcgagt tcgagggcgg gcgccttccc gtcctgggtc ttggcgatgt
     1741 actcggcgag gtcgttggcg tcgcgctcgg tctccagccg cttgaagtcg acgccgtgcc
     1801 ggtcgtcggg cgtgaaggcg gggttgacct tgcgcagggc ggcggtccac acggaccgcc
     1861 agtgcccctg ccactcgtcg agcgcggcgc cggtcggctc gaaggtggcg acgatctgct
     1921 tcgcggaccg ctccccctcg gtccggccgc cgaccaggac gatcgcgtgg atgtgcgggt
     1981 gccagccgtt gatctgcccc acggtgactt cggtcgcgcg gatcatgccg acgtacccga
     2041 tccggtctcg gatgccctcg cggtcggcgg cccggtgccc gtccttggcc cggcgtccgg
     2101 cccacgtgcc gcccgtgatc agtcgctggt aggcgcccgg ccgccggggg ctgtccggcg
     2161 tcttccgggt gccctggagg gcgtccatga ggtccgcgag ccggtccgtg tgcccatggc
     2221 gggccgtgaa ggtgaccagg taggcggtcc ccccgcgctt gatccactcg accacggcgg
     2281 cggtgatctc ctcggcccgc ttgtgccgga tcgtggcggc gcagaccggg cagagccaga
     2341 tccgcccgca ccgcatcagg cccaggacca cggacgttcc ggccgccgtc tgggcgacga
     2401 ttacgccgga ggcagggtcc atcagggcgc ggccgcagcc cttgcacgcg gcgtccccgc
     2461 tgatccgcca cagcgtccgg cggcggctgt accgggcggc tttccgcagt cgggcagcgt
     2521 cgctccgcga cgtgcttcct acttccgaga ggctgtcgcc tctcgggctc tccccatcca
     2581 ccccgtccgg agaaaccgca ggtcggaggg gtgcgggaaa ctctgttgtt tctttcccaa
     2641 ggtgttcgct tttgcctcgg gcggcatctc gcgtcacacg cgcgatcgcc cgcttcgctg
     2701 ccatccggca gcggtctgag cagtagatac gcggccgttt gcccggtgtg tgggcaattg
     2761 cggtcccgca gtggcagcgg ggcccggcgg gccgatctgg caatgcctcg gcatcgctcc
     2821 gtactctggg cacgagcaac gttcctgtct cgcccggcta aggggcgcga gtctgggagc
     2881 ggacgggtcg gaggtgcgaa gtccggcccg ttgctctttg gtctggtggg aatcctggca
     2941 ccaatcgggc cagaggttcc ctccgccact cccgacgccc cttggggctg gtgtgacttg
     3001 gagggccgaa gagagccccg ccggtgatcc ggcggggctt tgacgtgcgg tcagtgcgtg
     3061 tgtcggcgag cgatggccac gaggccctgg aagccgagcg gtccggcgaa gtcggcccag
     3121 tcgcaaccgg gctcagcgca gtgggcggac cagccaccgc cgttggggtc ctggaccagg
     3181 ttcacggtcc cctcggtcag gcgtccgtcg aagtcggtca tggtcggtct cctggtgggt
     3241 gggggcggtg cggtcggcgg cggcccagtc ctgctctcac gcaacgtcta cgtggacgct
     3301 cagggcgaca cgatcgaggt cggggagtcc gtcgctccgg cgggccgctg gcgcgtccac
     3361 cgggactgat caaggcgaat acttcatata tgcggggatc gaccgcgcgg gtcccggacg
     3421 gggaagagcg gggagctttg ccagagagcg acgacttccc cttgcgttgg tgattgccgg
     3481 tcagggcagc catccgccat cgtcgcgtag ggtgtcacac cccaggaatc gcgtcactga
     3541 acacagcagc cggtaggacg accatgactg agttggacac catcgcaaat ccgtccgatc
     3601 ccgcggtgca gcggatcatc gatgtcacca agccgtcgcg atccaacata aagacaacgt
     3661 tgatcgagga cgtcgagccc ctcatgcaca gcatcgcggc cggggtggag ttcatcgagg
     3721 tctacggcag cgacagcagt ccttttccat ctgagttgct ggatctgtgc gggcggcaga
     3781 acataccggt ccgcctcatc gactcctcga tcgtcaacca gttgttcaag ggggagcgga
     3841 aggccaagac attcggcatc gcccgcgtcc ctcgcccggc caggttcggc gatatcgcga
     3901 gccggcgtgg ggacgtcgtc gttctcgacg gggtgaagat cgtcgggaac atcggcgcga
     3961 tagtacgcac gtcgctcgcg ctcggagcgt cggggatcat cctggtcgac agtgacatca
     4021 ccagcatcgc ggaccggcgt ctccaaaggg ccagccgagg ttacgtcttc tcccttcccg
     4081 tcgttctctc cggtcgcgag gaggccatcg ccttcattcg ggacagcggt atgcagctga
     4141 tgacgctcaa ggcggatggc gacatttccg tgaaggaact cggggacaat ccggatcggc
     4201 tggccttgct gttcggcagc gaaaagggtg ggccttccga cctgttcgag gaggcgtctt
     4261 ccgcctcggt ttccatcccc atgatgagcc agaccgagtc tctcaacgtt tccgtttccc
     4321 tcggaatcgc gctgcacgag aggatcgaca ggaatctcgc ggccaaccga taagcgcctc
     4381 tgttcctcgg acgctcggtt cctcgacctc gattcgtcag tgatgatccc gcaaaagcgg
     4441 cctttgactc cctgcaagcc tcagcgaccg aatatatcgg ttatgcgtgg gcgatggttg
     4501 ttgtcattgt cggcgcaact atcggtatca agctgtttaa gaaattcacc tcgaaagcaa
     4561 gctgataaac cgatacaatt aaaggctcct tttggagcct ttttttttgg agattttcaa
     4621 cgtgaaaaaa ttattattcg caattccttt agttgttcct ttctattctc actccgctga
     4681 aactgttgaa agttgtttag caaaacctca tacagaaaat tcatttacta acgtctggaa
     4741 agacgacaaa actttagatc cggccaagct ctggcgtaat agcgaagagg cccgcaccga
     4801 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt
     4861 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4921 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4981 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5041 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5101 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5161 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5221 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     5281 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5341 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5401 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5461 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5521 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5581 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5641 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5701 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5761 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5821 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5881 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5941 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6001 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6061 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6121 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6181 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6241 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6301 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6361 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6601 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
//
